Linked Life Data

19358850

Source:http://linkedlifedata.com/resource/pubmed/id/19358850

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2009-6-29
pubmed:abstractText
Granulocytes and monocytes/macrophages represent key effector cells of the innate immune system. While human monocytes have been recognized as capable of secreting a broad spectrum of cytokines, the situation has been less clear in granulocytes with studies often showing conflicting results. In this study, lipopolysaccharide (LPS)-induced cytokine secretion from polymorphonuclear cells (PMN) and peripheral blood mononuclear cells (PBMC) was analyzed at the single cell level with the enzyme-linked immunospot (ELISpot) assay. This method allowed us to establish the cytokine profiles for both PBMC and PMN based on the frequency and pattern of cytokine secreting cells, rather than on the amount of produced cytokine detectable in solution by ELISA. As a result, low levels of contaminating mononuclear cells present in our PMN preparations could be discriminated from granulocytes. Using this technique, neutrophils were found to secrete the two chemokines, IL-8 and MIP-1beta in response to LPS. Also TNF-alpha was secreted but in lower amounts and by significantly fewer cells. However, and as opposed to several other reports, we were unable to detect secretion of IL-1beta, IL-6, IL-10, IL-12 and GM-CSF. In contrast to the limited cytokine production by PMN, PBMC secreted considerably larger amounts of the investigated cytokines with CD14(+) monocytes being the primary source of production. Finally, we believe that the cytokine ELISpot technique may provide a powerful tool by which cells of the innate immune system can be studied from a functional perspective at the single cell level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1872-7905
pubmed:author
pubmed:issnType
Electronic
pubmed:day
31
pubmed:volume
346
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-8
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
ELISpot analysis of LPS-stimulated leukocytes: human granulocytes selectively secrete IL-8, MIP-1beta and TNF-alpha.
pubmed:affiliation
Division of Infectious Diseases, Department of Medicine, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden. christian.smedman@ki.se
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't