Linked Life Data

15322069

Source:http://linkedlifedata.com/resource/pubmed/id/15322069

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-11-8
pubmed:abstractText
Bronchial asthma is characterized by chronic inflammation of airway tissues and nonspecific airway hyperresponsiveness (AHR), but the underlying mechanisms of AHR have yet to be elucidated. Recently, tumor necrosis factor-alpha (TNF-alpha) has been identified as a proinflammatory cytokine that might be important in the hyperresponsiveness of airway tissue. We have investigated the effects of SB-203580 (a p38 MAPK inhibitor), U-0126 (an inhibitor of p42/44 MAPK activation), and cycloheximide (an inhibitor of protein synthesis) on TNF-alpha-augmented ACh-induced bronchial smooth muscle contraction. We have also investigated the phosphorylation of p42/44 MAPK and upregulation of RhoA protein by TNF-alpha. Treatment of rat bronchial smooth muscles with TNF-alpha (300 and 1,000 ng/ml for 24 h) resulted in a significant upward shift in the concentration-response curve to ACh, but not to high K(+), compared with control tissues. The effect of TNF-alpha was completely blocked by pretreatment with U-0126 or cycloheximide, but not with SB-203580. Immunoblotting demonstrated that p42/44 MAPK was phosphorylated and RhoA protein was increased in bronchial tissue by TNF-alpha. Furthermore, the TNF-alpha-induced upregulation of RhoA protein was abolished by U-0126 pretreatment. In conclusion, we suggest that TNF-alpha might be one of the important mediators involved in the pathogenesis of augmented bronchial smooth muscle contractility in AHR. For the first time, we have demonstrated that augmentation of ACh-induced contractile response evoked by TNF-alpha was mediated by synthesis of protein, such as RhoA, through activation of p42/44, but not p38 MAPK, in rat bronchial smooth muscle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
8750-7587
pubmed:author
pubmed:issnType
Print
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2154-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:15322069-Acetylcholine, pubmed-meshheading:15322069-Animals, pubmed-meshheading:15322069-Blotting, Western, pubmed-meshheading:15322069-Bronchi, pubmed-meshheading:15322069-MAP Kinase Signaling System, pubmed-meshheading:15322069-Male, pubmed-meshheading:15322069-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:15322069-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:15322069-Muscle, Smooth, pubmed-meshheading:15322069-Muscle Contraction, pubmed-meshheading:15322069-Phosphorylation, pubmed-meshheading:15322069-Rats, pubmed-meshheading:15322069-Rats, Wistar, pubmed-meshheading:15322069-Specific Pathogen-Free Organisms, pubmed-meshheading:15322069-Tumor Necrosis Factor-alpha, pubmed-meshheading:15322069-Up-Regulation, pubmed-meshheading:15322069-rhoA GTP-Binding Protein
pubmed:year
2004
pubmed:articleTitle
Involvement of p42/44 MAPK and RhoA protein in augmentation of ACh-induced bronchial smooth muscle contraction by TNF-alpha in rats.
pubmed:affiliation
Dept. of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't