Linked Life Data

9714185

Source:http://linkedlifedata.com/resource/pubmed/id/9714185

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1998-8-31
pubmed:abstractText
The production of monocyte chemoattractant protein-1 (MCP-1) and its regulation by TNFalpha, IL-1, and IL-8 were investigated in two rabbit models of arthritis induced by intra-articular injection of lipopolysaccharide (LPS) or monosodium urate (MSU) crystals. We first prepared recombinant rabbit MCP-1 and antibodies and then developed an immunoassay. The immunoassay detected 3 pg/ml rabbit MCP-1 and did not cross-react with other rabbit chemokines such as IL-8 or GRO. MCP-1 was first detected in synovial fluid (SF) at 1 hour, and peaked at 4 or 2 hours after the injection of LPS or MSU crystals, respectively. Immunohistochemically, MCP-1 was detected in synovial lining cells and infiltrating neutrophils. The amounts of MCP-1 detected in SF from neutrophil-depleted rabbits were similar to those in normal rabbits, suggesting that synovial lining cells were the main source of MCP-1 detected in SF. The peak level of MCP-1 in SF after LPS-injection was inhibited by 57% with anti-TNFalpha mAb and by 41% with IL-1 receptor antagonist (IL-1Ra). Coadministration of anti-TNFalpha mAb and IL-1Ra inhibited 90% of MCP-1 production. In contrast, the peak level of MCP-1 in SF after MSU crystal-injection was not affected by any cytokine inhibitor, but was reduced by 52% with coadministration of anti-TNFalpha mAb and IL-1Ra. Anti-IL-8 IgG had no effect on the production of MCP-1 in either model. Thus, the production of MCP-1 in LPS-induced arthritis was mostly regulated by TNFalpha and IL-1, whereas half the extent of MCP-1 production in MSU crystal-induced arthritis was independent of TNFalpha or IL-1. IL-8 does not seem to regulate the production of MCP-1 in SF either directly or indirectly. Finally, administration of neutralizing anti-MCP-1 antibody inhibited LPS- and MSU crystal-induced monocyte infiltration by 58.4% and 44.9%, respectively, suggesting that synovial production of MCP-1 plays an important role in the recruitment of monocytes in these arthritis models.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
973-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9714185-Animals, pubmed-meshheading:9714185-Antibodies, Monoclonal, pubmed-meshheading:9714185-Arthritis, pubmed-meshheading:9714185-COS Cells, pubmed-meshheading:9714185-Cell Movement, pubmed-meshheading:9714185-Chemokine CCL2, pubmed-meshheading:9714185-Crystallization, pubmed-meshheading:9714185-Female, pubmed-meshheading:9714185-Fluoroimmunoassay, pubmed-meshheading:9714185-Immune Sera, pubmed-meshheading:9714185-Injections, Intra-Articular, pubmed-meshheading:9714185-Injections, Intravenous, pubmed-meshheading:9714185-Interleukin-1, pubmed-meshheading:9714185-Interleukin-8, pubmed-meshheading:9714185-Knee Joint, pubmed-meshheading:9714185-Lipopolysaccharides, pubmed-meshheading:9714185-Monocytes, pubmed-meshheading:9714185-Rabbits, pubmed-meshheading:9714185-Recombinant Proteins, pubmed-meshheading:9714185-Synovial Fluid, pubmed-meshheading:9714185-Tumor Necrosis Factor-alpha, pubmed-meshheading:9714185-Uric Acid
pubmed:year
1998
pubmed:articleTitle
Production and regulation of monocyte chemoattractant protein-1 in lipopolysaccharide- or monosodium urate crystal-induced arthritis in rabbits: roles of tumor necrosis factor alpha, interleukin-1, and interleukin-8.
pubmed:affiliation
Department of Pathology, Kumamoto University School of Medicine, Honjo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't