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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6686
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pubmed:dateCreated |
1998-7-1
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pubmed:databankReference |
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pubmed:abstractText |
The entry of human immunodeficiency virus (HIV) into cells requires the sequential interaction of the viral exterior envelope glycoprotein, gp120, with the CD4 glycoprotein and a chemokine receptor on the cell surface. These interactions initiate a fusion of the viral and cellular membranes. Although gp120 can elicit virus-neutralizing antibodies, HIV eludes the immune system. We have solved the X-ray crystal structure at 2.5 A resolution of an HIV-1 gp120 core complexed with a two-domain fragment of human CD4 and an antigen-binding fragment of a neutralizing antibody that blocks chemokine-receptor binding. The structure reveals a cavity-laden CD4-gp120 interface, a conserved binding site for the chemokine receptor, evidence for a conformational change upon CD4 binding, the nature of a CD4-induced antibody epitope, and specific mechanisms for immune evasion. Our results provide a framework for understanding the complex biology of HIV entry into cells and should guide efforts to intervene.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
393
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
648-59
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9641677-Amino Acid Sequence,
pubmed-meshheading:9641677-Animals,
pubmed-meshheading:9641677-Antigens, CD4,
pubmed-meshheading:9641677-CHO Cells,
pubmed-meshheading:9641677-Cricetinae,
pubmed-meshheading:9641677-Cricetulus,
pubmed-meshheading:9641677-Crystallography, X-Ray,
pubmed-meshheading:9641677-Glycosylation,
pubmed-meshheading:9641677-HIV Antibodies,
pubmed-meshheading:9641677-HIV Envelope Protein gp120,
pubmed-meshheading:9641677-HIV Envelope Protein gp41,
pubmed-meshheading:9641677-Humans,
pubmed-meshheading:9641677-Immunoglobulin Fragments,
pubmed-meshheading:9641677-Membrane Fusion,
pubmed-meshheading:9641677-Models, Molecular,
pubmed-meshheading:9641677-Molecular Sequence Data,
pubmed-meshheading:9641677-Neutralization Tests,
pubmed-meshheading:9641677-Protein Conformation,
pubmed-meshheading:9641677-Receptors, CCR5
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pubmed:year |
1998
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pubmed:articleTitle |
Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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