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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6 Pt 1
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pubmed:dateCreated |
1997-8-18
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pubmed:abstractText |
Osteogenesis is a complex process whereby growth factors and mediators from both local and systemic sources modulate the bone-forming activities of osteoblasts. In the present study we utilized primary cultures of fetal rat calvarial cells to characterize osteoblast responsiveness to the vascular mediator endothelin-1 (ET-1) and to investigate whether ET-1 responses are regulated by osteogenic protein-1 (OP-1). We found that a 1- to 2-day exposure to OP-1 diminished ET-1 receptor ligand binding and signal transduction by downregulating ET-1 receptor mRNA expression. ET-1-mediated calcium signaling and ligand binding were completely abolished by the ETA receptor antagonist BQ-123, suggesting that ET-1 effects are mediated by this receptor. Northern analysis of total RNA revealed that ETA mRNA expression was inhibited approximately 50% by OP-1 treatment, whereas ETB receptor mRNA was not detected by this method of analysis. In OP-1-treated cultures, the magnitude and duration of ET-1 calcium signals varied among individual cells. This finding may be related to a heterogeneous OP-1 response, indicated by alkaline phosphatase induction in only a subpopulation of cells. These results suggest that modulation of osteoblast function by ET-1 occurs during distinct periods of phenotypic development and imply that downregulation of ET-1 responsiveness may be necessary for optimal bone formation in vivo.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/BMP7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Bmp7 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 7,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides, Cyclic,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Endothelin A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Endothelin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/cyclo(Trp-Asp-Pro-Val-Leu)
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
E967-75
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9227439-Alkaline Phosphatase,
pubmed-meshheading:9227439-Animals,
pubmed-meshheading:9227439-Bone Morphogenetic Protein 7,
pubmed-meshheading:9227439-Bone Morphogenetic Proteins,
pubmed-meshheading:9227439-Calcium,
pubmed-meshheading:9227439-Cells, Cultured,
pubmed-meshheading:9227439-Cytosol,
pubmed-meshheading:9227439-Down-Regulation,
pubmed-meshheading:9227439-Endothelin-1,
pubmed-meshheading:9227439-Fetus,
pubmed-meshheading:9227439-Humans,
pubmed-meshheading:9227439-Kinetics,
pubmed-meshheading:9227439-Osteoblasts,
pubmed-meshheading:9227439-Peptides, Cyclic,
pubmed-meshheading:9227439-Phosphatidylinositol Diacylglycerol-Lyase,
pubmed-meshheading:9227439-Rats,
pubmed-meshheading:9227439-Rats, Sprague-Dawley,
pubmed-meshheading:9227439-Receptor, Endothelin A,
pubmed-meshheading:9227439-Receptors, Endothelin,
pubmed-meshheading:9227439-Recombinant Proteins,
pubmed-meshheading:9227439-Skull,
pubmed-meshheading:9227439-Transforming Growth Factor beta,
pubmed-meshheading:9227439-Type C Phospholipases
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pubmed:year |
1997
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pubmed:articleTitle |
Osteogenic protein-1 downregulates endothelin A receptors in primary rat osteoblasts.
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pubmed:affiliation |
Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284-7760, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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