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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-2-16
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pubmed:abstractText |
To identify the signals involved in the adhesion and subsequent migration of lymphocytes across the endothelium (REC) and pigment epithelium (RPE) of the blood-retina barrier we have studied the effects of monoclonal antibodies (mAb) to rat adhesion/accessory molecules on the binding of normal and concanavalin A (Con A)-activated rat spleen lymphocytes to cultured unstimulated and interferon-gamma (IFN-gamma)-stimulated RPE and REC. Forty to 48% of unactivated T cells were found to bind to normal REC or RPE by leucocyte function-associated antigen-1/intercellular adhesion molecule-1 (LFA-1/ICAM-1)-independent mechanisms, despite constitutive expression of ICAM-1 by the RPE cells and LFA-1 by the T cells. Con A-activated lymphocytes showed an enhanced adhesion to both RPE and REC. However, IFN-gamma-stimulated RPE and REC did not demonstrate a significant increase in adhesiveness for normal lymphocytes highlighting the importance of lymphocyte integrin activation from low-affinity to high-affinity state. Activated lymphocyte adhesion to unstimulated RPE and REC was significantly blocked by LFA-1 mAb (35%, P < 0.0001) and ICAM-1 mAb (20%, P < 0.001). Inhibition of adhesion by antibody to CD2 was not significant. Both ICAM-1 and LFA-1 mAb also significantly (P < 0.05) blocked antigen presentation following retinal extract stimulation of lymphocytes from immunized rats in proliferation assay. These data suggest that the ICAM-1/LFA-1 system is important in lymphocyte trafficking into the eye only after lymphocyte activation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1283598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1286702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1346139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1347662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1374611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1672652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1692079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1716919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1950797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-1980120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-2107983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-2185780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-2653373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-2951597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-3028640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-3069283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-3259610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-3406938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-3517218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-3525675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-4055292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-4587740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-8095510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-8100190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-8330312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7821966-8457510
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
52-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7821966-Animals,
pubmed-meshheading:7821966-Antibodies, Monoclonal,
pubmed-meshheading:7821966-Blood-Retinal Barrier,
pubmed-meshheading:7821966-Cell Adhesion,
pubmed-meshheading:7821966-Cells, Cultured,
pubmed-meshheading:7821966-Concanavalin A,
pubmed-meshheading:7821966-Endothelium, Vascular,
pubmed-meshheading:7821966-Histocompatibility Antigens Class II,
pubmed-meshheading:7821966-Intercellular Adhesion Molecule-1,
pubmed-meshheading:7821966-Interferon-gamma,
pubmed-meshheading:7821966-Lymphocyte Activation,
pubmed-meshheading:7821966-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:7821966-Pigment Epithelium of Eye,
pubmed-meshheading:7821966-Rats,
pubmed-meshheading:7821966-Rats, Inbred Strains,
pubmed-meshheading:7821966-Retina,
pubmed-meshheading:7821966-T-Lymphocytes
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pubmed:year |
1994
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pubmed:articleTitle |
ICAM-1/LFA-1 interactions in T-lymphocyte activation and adhesion to cells of the blood-retina barrier in the rat.
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pubmed:affiliation |
Department of Ophthalmology, University of Aberdeen Medical School, Foresterhill, UK.
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pubmed:publicationType |
Journal Article
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