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rdf:type |
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lifeskim:mentions |
umls-concept:C0027882,
umls-concept:C0031180,
umls-concept:C0078939,
umls-concept:C0330390,
umls-concept:C0332120,
umls-concept:C0596235,
umls-concept:C0599946,
umls-concept:C0600688,
umls-concept:C0611285,
umls-concept:C1314939,
umls-concept:C1456820,
umls-concept:C1521761
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pubmed:issue |
20
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pubmed:dateCreated |
1995-10-27
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pubmed:abstractText |
In Alzheimer disease (AD) the amyloid beta-peptide (A beta) accumulates in plaques in the brain. A beta can be neurotoxic by a mechanism involving induction of reactive oxygen species (ROS) and elevation of intracellular free calcium levels ([Ca2+]i). In light of evidence for an inflammatory response in the brain in AD and reports of increased levels of tumor necrosis factor (TNF) in AD brain we tested the hypothesis that TNFs affect neuronal vulnerability to A beta. A beta-(25-35) and A beta-(1-40) induced neuronal degeneration in a concentration- and time-dependent manner. Pretreatment of cultures for 24 hr with TNF-beta or TNF-alpha resulted in significant attenuation of A beta-induced neuronal degeneration. Accumulation of peroxides induced in neurons by A beta was significantly attenuated in TNF-pretreated cultures, and TNFs protected neurons against iron toxicity, suggesting that TNFs induce antioxidant pathways. The [Ca2+]i response to glutamate (quantified by fura-2 imaging) was markedly potentiated in neurons exposed to A beta, and this action of A beta was suppressed in cultures pretreated with TNFs. Electrophoretic mobility-shift assays demonstrated an induction of a kappa beta-binding activity in hippocampal cells exposed to TNFs. Exposure of cultures to I kappa B (MAD3) antisense oligonucleotides, a manipulation designed to induce NF-kappa B, mimicked the protection by TNFs. These data suggest that TNFs protect hippocampal neurons against A beta toxicity by suppressing accumulation of ROS and Ca2+ and that kappa B-dependent transcription is sufficient to mediate these effects. A modulatory role for TNF in the neurodegenerative process in AD is proposed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1346802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1362341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1371849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1473734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1611474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1745413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1829460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1849278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-1860055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-2289145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-2508093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-2529544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7507336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7518651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7541884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7715705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7835247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7857652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7884819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7901908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-7982093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8004671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8014208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8023366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8034754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8035184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8061327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8070512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8097519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8122310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8137429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8159737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8181053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8221072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8335941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8423065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7568127-8439410
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid beta-Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphotoxin-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxides,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9328-32
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7568127-Humans,
pubmed-meshheading:7568127-Animals,
pubmed-meshheading:7568127-Mice,
pubmed-meshheading:7568127-Calcium,
pubmed-meshheading:7568127-Rats,
pubmed-meshheading:7568127-Glutamic Acid,
pubmed-meshheading:7568127-Neurons,
pubmed-meshheading:7568127-Embryo, Mammalian,
pubmed-meshheading:7568127-Peroxides,
pubmed-meshheading:7568127-Peptide Fragments,
pubmed-meshheading:7568127-Reactive Oxygen Species,
pubmed-meshheading:7568127-Kinetics,
pubmed-meshheading:7568127-Base Sequence,
pubmed-meshheading:7568127-Cells, Cultured,
pubmed-meshheading:7568127-Binding Sites,
pubmed-meshheading:7568127-Molecular Sequence Data,
pubmed-meshheading:7568127-Mice, Inbred C57BL
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