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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1986-7-3
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pubmed:abstractText |
Drosophila melanogaster larvae reared on food containing radioactive cadmium retained over 80% of it, mostly in the intestinal epithelium. The majority of this radioactivity was associated with a soluble protein of less than 10,000 molecular weight. Synthesis of this cadmium-binding protein was induced by the metal as demonstrated by incorporation of radioactive cysteine. Most copper ingested by larvae was also found to associate with a low molecular weight, inducible protein, but some of it was found in an insoluble fraction. Zinc was unable to, or very inefficient at, binding or inducing the synthesis of a similar protein. A D. melanogaster cDNA clone was isolated based on its more intense hybridization to copies of RNA sequences from copper-fed larvae than from control larvae. This clone showed strong hybridization to mouse metallothionein-I cDNA at reduced stringency. Its nucleotide sequence includes an open-reading segment which codes for a 40-amino acid protein; this protein was identified as metallothionein based on its similarity to the amino-terminal portion of mammalian and crab metalloproteins. The ten cysteine residues present occur in five pairs of near-vicinal cysteines (Cys-X-Cys). This cDNA sequence hybridized to a 400-nucleotide polyadenylated RNA whose presence in the cells of the alimentary canal of larvae was stimulated by ingestion of cadmium or copper; in other tissues this RNA was present at much lower levels. Mercury, silver, and zinc induced metallothionein to a lesser extent. Whether (any of) the protein(s) discussed above correspond(s) to that coded by this RNA sequence has not yet been determined.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-16589200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-2578462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-411798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-452022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-6243777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-6320108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-6816669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-6896577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-6907091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-6935664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-7061431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-7263772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-743236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-805154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3086075-898179
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0091-6765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
107-16
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3086075-Amino Acid Sequence,
pubmed-meshheading:3086075-Animals,
pubmed-meshheading:3086075-Cadmium,
pubmed-meshheading:3086075-Cloning, Molecular,
pubmed-meshheading:3086075-Copper,
pubmed-meshheading:3086075-Cysteine,
pubmed-meshheading:3086075-DNA,
pubmed-meshheading:3086075-Drosophila melanogaster,
pubmed-meshheading:3086075-Gene Expression Regulation,
pubmed-meshheading:3086075-Larva,
pubmed-meshheading:3086075-Lysosomes,
pubmed-meshheading:3086075-Metallothionein,
pubmed-meshheading:3086075-Silver,
pubmed-meshheading:3086075-Zinc
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pubmed:year |
1986
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pubmed:articleTitle |
Effects of heavy metals on Drosophila larvae and a metallothionein cDNA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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