Linked Life Data

2786182

Source:http://linkedlifedata.com/resource/pubmed/id/2786182

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-7-10
pubmed:abstractText
Blood monocytes produce two well-defined cytokines, tumor necrosis factor/cachectin (TNF) and Il-1, that have multiple immunologic and inflammatory functions. This study examined the secretion of these cytokines by cord blood monocytes from preterm and term neonates stimulated with or without lipopolysaccharide (LPS). Seventeen samples (eight preterm, nine term) were collected. Supernatants of monocytes were assayed for activities of IL-1 (mitogenesis for mouse thymocytes) and TNF (cytotoxicity for L929 cells). IL-1 and TNF activities were not significant in supernatants of unstimulated monocytes from either preterm or term infants. IL-1 secretion by LPS-stimulated monocytes from term and preterm neonates was comparable to IL-1 activity by monocytes from adults, but TNF activity by LPS-stimulated monocytes from preterm neonates was significantly less than that from monocytes of either term or adult groups (p less than 0.05). TNF activity in supernatants of LPS-stimulated monocytes was neutralized 100% by monoclonal antibody to TNF-alpha; IL-1 activity was neutralized 80% by polyclonal antiserum to IL-1-beta. Given the multifactorial biologic activities of TNF, the decreased secretion of TNF by monocytes from preterm neonates may be significant in describing mechanisms for the increased susceptibility of the preterm neonate to infection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0031-3998
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
342-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Tumor necrosis factor/cachectin and interleukin-1 secretion by cord blood monocytes from premature and term neonates.
pubmed:affiliation
Department of Neonatology, Rainbow Babies and Children's Hospital, Cleveland, Ohio.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.