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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1989-8-4
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pubmed:abstractText |
Forty-one human tumour cell lines were examined for the production of epidermal growth factor (EGF)/transforming growth factor (TGF)-alpha-like activity (EGF/TGF-alpha-LA), immunoreactive (IR-) EGF and IR-TGF-alpha. EGF/TGF-alpha-LA was determined by radioreceptor assay, in which the factors with capacity to bind to EGF receptor could be detected. IR-EGF and IR-TGF-alpha were determined by the respective radioimmunoassays. Both EGF/TGF-alpha-LA and IR-TGF-alpha were detected in 11 tumour cell lines. The levels of EGF/TGF-alpha-LA correlated well with those of IR-TGF-alpha. A small amount of IR-TGF-alpha was detected in five other lines. In contrast, IR-EGF was not detectable in any of the 41. Consequently, it can be concluded that EGF/TGF-alpha-LA produced by human tumour cells is mainly TGF-alpha rather than EGF. It was also revealed that melanoma cell lines produce a large amount of TGF-alpha frequently. Gel filtration studies revealed that TGF-alpha produced by melanoma cell lines was identical to human (h) TGF-alpha(1-50), except for one line, in which IR-TGF-alpha with a different molecular size was detected. Northern blot analysis revealed that bands corresponding to hTGF-alpha mRNA were present in melanoma cell lines producing a large amount of IR-TGF-alpha, indicating that the TGF-alpha produced is the product of hTGF-alpha gene. Further studies are required to discover the actual biological roles of TGF-alpha produced by melanoma cells as well as other types of cancer cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-14450081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-2442615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3022030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3039200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3137193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3163273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3260816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3300642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3465381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3467839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3467848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3491676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3499610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3574460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3855503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-3962675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-4094096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-6088071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-6090450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-6283553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-6308641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-6771000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2736210-6982383
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
761-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2736210-Blotting, Northern,
pubmed-meshheading:2736210-Cell Line,
pubmed-meshheading:2736210-Chromatography, Gel,
pubmed-meshheading:2736210-Humans,
pubmed-meshheading:2736210-Melanoma,
pubmed-meshheading:2736210-Neoplasms,
pubmed-meshheading:2736210-Radioimmunoassay,
pubmed-meshheading:2736210-Radioligand Assay,
pubmed-meshheading:2736210-Transforming Growth Factors,
pubmed-meshheading:2736210-Tumor Cells, Cultured
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pubmed:year |
1989
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pubmed:articleTitle |
Production of transforming growth factor-alpha in human tumour cell lines.
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pubmed:affiliation |
Growth Factor Division, National Cancer Research Institute, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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