Linked Life Data

17609194

Source:http://linkedlifedata.com/resource/pubmed/id/17609194

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-7-4
pubmed:abstractText
This study aims to investigate the expression of metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in chronic doxorubicin cardiomyopathy in a rabbit model and to evaluate the effects of bone marrow-derived mesenchymal stem cell (MSC) transplantation in this disease. Thirty-nine 3-month-old New Zealand rabbits were divided into 4 groups: group 1 (n = 9) was the untreated control. Groups 2-4 were treated with 6 weeks of doxorubicin (3 mg/kg). Group 2 (n = 6) received no further treatment. In group 3 (n = 9), animals were treated with culture medium (CM) alone. In group 4 (n = 15), autologous MSCs (1.5-2.0 x 10(6)/ml) were injected in the left ventricular (LV) wall. Hearts were stained with HE and picrosirius red. MMP-1, -2, -3 and -9 and TIMP-2 and -3 were detected immunohistochemically. The mRNA levels were determined by real-time polymerase chain reaction. The results confirmed that doxorubicin treatment resulted in minimal myocardial fibrosis and showed that expression of MMPs increased and TIMP-3 decreased. The injection procedure resulted in increased myocardial fibrosis in groups 3 and 4. After MSC injection, MMP-1, MMP-2, and TIMP-3 expression was higher than that in group 2. CM injection led to more fibrosis, elevated TIMP-3, but diminished MMP-1 and MMP-2 expression compared with MSC injection. The mRNA levels of MMPs and TIMPs were not significantly different among all groups. In conclusion, chronic doxorubicin cardiomyopathy was characterized by increased MMP and decreased TIMP-3 expression. MSCs injection into the LV resulted in marked differences of collagen content and MMP/TIMP expression in the whole heart, although significant numbers of living MSCs were not detected after 4 weeks.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0300-9858
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
494-503
pubmed:meshHeading
pubmed-meshheading:17609194-Animals, pubmed-meshheading:17609194-Antibiotics, Antineoplastic, pubmed-meshheading:17609194-Cardiomyopathies, pubmed-meshheading:17609194-Doxorubicin, pubmed-meshheading:17609194-Drug Administration Schedule, pubmed-meshheading:17609194-Gene Expression Regulation, Enzymologic, pubmed-meshheading:17609194-Immunohistochemistry, pubmed-meshheading:17609194-Male, pubmed-meshheading:17609194-Mesenchymal Stem Cells, pubmed-meshheading:17609194-Metalloproteases, pubmed-meshheading:17609194-Models, Animal, pubmed-meshheading:17609194-Myocardium, pubmed-meshheading:17609194-RNA, Messenger, pubmed-meshheading:17609194-Rabbits, pubmed-meshheading:17609194-Stem Cell Transplantation, pubmed-meshheading:17609194-Tissue Inhibitor of Metalloproteinases, pubmed-meshheading:17609194-Transplantation, Autologous
pubmed:year
2007
pubmed:articleTitle
Effects of autologous stem cells on immunohistochemical patterns and gene expression of metalloproteinases and their tissue inhibitors in doxorubicin cardiomyopathy in a rabbit model.
pubmed:affiliation
Institut für Veterinär-Pathologie, An den Tierkliniken 33, 04103 Leipzig, Germany. aupperle@rz.uni-leipzig.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't