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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
30
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pubmed:dateCreated |
1991-12-2
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pubmed:abstractText |
The chimeric EK-receptor (EK-R), consisting of the epidermal growth factor receptor (EGF-R) extracellular binding domain and p145c-kit cytoplasmic signal-generating sequences, was fully functional in forming high and low affinity EGF binding sites and in ligand-regulated receptor and substrate phosphorylation activities. Relative to EGF-R, EK-R activation stimulated kit-characteristic phosphorylation of human 293 fibroblast substrate polypeptides. Transient coexpression of EK-R with candidate substrates resulted in ligand-induced phosphorylation of phospholipase C gamma and guanosine triphosphatase-activating polypeptide. The RAF-1 serine/threonine kinase was shown to be associated with activated EK-R, but no tyrosine phosphorylation could be detected. The faithfulness of EK-R substrate phosphorylation specificity was confirmed with stem cell factor-stimulated p145c-kit.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
266
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19908-16
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1718957-Amino Acid Sequence,
pubmed-meshheading:1718957-Base Sequence,
pubmed-meshheading:1718957-Cells, Cultured,
pubmed-meshheading:1718957-Chimera,
pubmed-meshheading:1718957-Epidermal Growth Factor,
pubmed-meshheading:1718957-GTPase-Activating Proteins,
pubmed-meshheading:1718957-Gene Expression,
pubmed-meshheading:1718957-Humans,
pubmed-meshheading:1718957-Molecular Sequence Data,
pubmed-meshheading:1718957-Phosphorylation,
pubmed-meshheading:1718957-Precipitin Tests,
pubmed-meshheading:1718957-Protein-Tyrosine Kinases,
pubmed-meshheading:1718957-Proteins,
pubmed-meshheading:1718957-Proto-Oncogene Proteins,
pubmed-meshheading:1718957-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:1718957-Receptors, Cell Surface,
pubmed-meshheading:1718957-Substrate Specificity,
pubmed-meshheading:1718957-Type C Phospholipases
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pubmed:year |
1991
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pubmed:articleTitle |
Substrate phosphorylation specificity of the human c-kit receptor tyrosine kinase.
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pubmed:affiliation |
Department of Molecular Biology, Max-Planck-Institut für Biochemie, Martinsried, Germany.
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pubmed:publicationType |
Journal Article
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