Linked Life Data

10047952

Source:http://linkedlifedata.com/resource/pubmed/id/10047952

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-3-22
pubmed:abstractText
Researchers working with stable isotopes are faced with the problem of preserving animal samples without altering their isotope ratios. We evaluated the effects of several preservation treatments on the isotopic content of Drosophila samples. Results show that, when there is a danger of rotting, preservation treatment is indispensable to preserve intact isotope ratios, but that not all treatments are equally appropriate. The best is freezing without liquid nitrogen. Ethanol or ethylene glycol preserve the delta 15N, but change the delta 13C. Formalin should be avoided. Storage in a NaCl solution may be a good short-term alternative to freezing for both elements. In the control males and females, the trophic isotopic shift was, respectively, +2.9 and +2.6/1000 for delta 15N, and +1.1/1000 for delta 13C (no difference between sexes). Despite a standardised rearing protocol, there were small but significant inter-tube differences, caused by microenvironmental rather than by genetic factors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0764-4469
pubmed:author
pubmed:issnType
Print
pubmed:volume
322
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Effects of several preservation methods on the isotopic content of Drosophila samples.
pubmed:affiliation
Ecologie des populations et communautés, Ura 2154 du CNRS-université Paris-XI, Orsay, France. sergine.ponsard@epc.u-psud.fr
pubmed:publicationType
Journal Article, Comparative Study