Linked Life Data

9756413

Source:http://linkedlifedata.com/resource/pubmed/id/9756413

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1998-10-15
pubmed:abstractText
We have previously illustrated the importance of B7-2 expression for the enhanced generation of cytotoxic T lymphocyte (CTL) activity by stimulation cultures of tumor bearer splenic cells to which tumor necrosis factor alpha (TNFalpha) has been added. Here we show that the B7-1 molecule is also important for CTL generation by such stimulation cultures, although to a much lesser extent than the B7-2 molecule. In addition, we show the importance of CD40/CD40L interaction for the expression of the B7-2 molecule, but not the B7-1 molecule, by tumor bearer splenic cells stimulated in vitro in the presence of TNF. The CD40/CD40L interaction is also shown to be important for the generation of CTL activity by tumor bearer splenic cells stimulated in vitro in the presence of exogenous TNF. However, the CD40/CD40L interaction is less important for the generation of enhanced CTL activity than for the expression of an elevated level of B7-2. Specifically, blockade of CD40/CD40L interaction, which reduced the level of B7-2 expressed by tumor bearer splenic cells stimulated in vitro in the presence of TNF to the level of B7-2 expressed by tumor bearer splenic cells stimulated in vitro in the absence of exogenous TNF, failed to reduce the level of CTL generated to the level generated by tumor bearer splenic cells stimulated in the absence of exogenous TNF. Finally, blockade of CD40/CD40L interaction was inferior to blockade of B7-2/CD28 interaction in inhibiting the generation of CTL activity by tumor bearer splenic cells stimulated in the presence of exogenous TNF. Thus, although CD40/CD40L interaction is important for the generation of enhanced CTL activity by stimulation cultures of tumor bearer splenic cells to which TNF has been added, TNF also mediates its potentiating effect for CTL generation by such stimulation cultures via other mechanisms that are independent of CD40/CD40L interaction but dependent on B7-2 expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0340-7004
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
293-303
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9756413-Animals, pubmed-meshheading:9756413-Antibodies, Monoclonal, pubmed-meshheading:9756413-Antigens, CD, pubmed-meshheading:9756413-Antigens, CD28, pubmed-meshheading:9756413-Antigens, CD40, pubmed-meshheading:9756413-Antigens, CD80, pubmed-meshheading:9756413-Antigens, CD86, pubmed-meshheading:9756413-CD40 Ligand, pubmed-meshheading:9756413-Cells, Cultured, pubmed-meshheading:9756413-Cytotoxicity, Immunologic, pubmed-meshheading:9756413-Female, pubmed-meshheading:9756413-Macromolecular Substances, pubmed-meshheading:9756413-Membrane Glycoproteins, pubmed-meshheading:9756413-Mice, pubmed-meshheading:9756413-Mice, Inbred BALB C, pubmed-meshheading:9756413-Plasmacytoma, pubmed-meshheading:9756413-Spleen, pubmed-meshheading:9756413-T-Lymphocytes, Cytotoxic, pubmed-meshheading:9756413-Tumor Necrosis Factor-alpha
pubmed:year
1998
pubmed:articleTitle
Limited importance of CD40/CD40L interaction in the B7-dependent generation of anti-MOPC-315 cytotoxic T lymphocyte activity by tumor bearer splenic cells stimulated in vitro in the presence of tumor necrosis factor.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Illinois at Chicago, 60612, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't