Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1998-11-18
|
| pubmed:abstractText |
The release of cytokines from cutaneous cells may be of major importance in the initiation and development of many inflammatory skin disorders. For example, tumor necrosis factor-alpha (TNF-alpha), which in healthy skin is found preformed only in mast cells, is able to induce the expression of several adhesion molecules including intercellular adhesion molecule-1 (ICAM-1). Increased expression of ICAM-1 occurs in keratinocytes in lesional skin of psoriasis and atopic dermatitis (AD) and it is considered to be an important initiator of leucocyte/keratinocyte interactions in skin inflammation. We counted the mast cells showing TNF-alpha immunoreactivity using a double-staining method in nonlesional and lesional skin sections from 12 patients with AD and 12 patients with psoriasis. The percentage of TNF-alpha+ mast cells in lesional and nonlesional AD skin was 36 +/- 22% and 21 +/- 15% (P < 0.018, paired t-test), respectively, and in psoriatic skin was 16 +/- 25% and 15 +/- 15%, respectively (P < 0.89, paired t-test). We also cultured whole skin biopsies taken from the healthy-looking skin of psoriatic and AD patients in the presence of mast cell degranulator compound 48/80, which resulted in focal expression of ICAM-1 in the epidermis. In cultured keratinocytes, both histamine and an extract of a human mast-cell line (HMC-1) induced ICAM-1 immunostaining only in occasional cells, but the combination of histamine and the HMC-1 extract resulted in intense ICAM-1 staining in numerous cells. This enhancement of ICAM-1 staining was abolished by preincubation of the HMC-1 extract with anti-TNF-alpha antibody. These results suggest that the degranulation of mast cells induces the expression of ICAM-1 in keratinocytes probably via TNF-alpha and histamine.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0340-3696
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
290
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
353-9
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9749989-Adult,
pubmed-meshheading:9749989-Aged,
pubmed-meshheading:9749989-Antibodies, Monoclonal,
pubmed-meshheading:9749989-Biopsy,
pubmed-meshheading:9749989-Cytoplasmic Granules,
pubmed-meshheading:9749989-Dermatitis, Atopic,
pubmed-meshheading:9749989-Epidermis,
pubmed-meshheading:9749989-Female,
pubmed-meshheading:9749989-Histamine,
pubmed-meshheading:9749989-Humans,
pubmed-meshheading:9749989-Intercellular Adhesion Molecule-1,
pubmed-meshheading:9749989-Keratinocytes,
pubmed-meshheading:9749989-Male,
pubmed-meshheading:9749989-Mast Cells,
pubmed-meshheading:9749989-Middle Aged,
pubmed-meshheading:9749989-Psoriasis,
pubmed-meshheading:9749989-Skin,
pubmed-meshheading:9749989-Tumor Cells, Cultured,
pubmed-meshheading:9749989-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Mast cells of psoriatic and atopic dermatitis skin are positive for TNF-alpha and their degranulation is associated with expression of ICAM-1 in the epidermis.
|
| pubmed:affiliation |
Department of Dermatology, University of Kuopio, Finland.
|
| pubmed:publicationType |
Journal Article
|