Linked Life Data

9349574

Source:http://linkedlifedata.com/resource/pubmed/id/9349574

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-11-13
pubmed:abstractText
Matrix metalloproteinase-1 (MMP-1) and tissue matrix metalloproteinase inhibitor 1 (TIMP-1) play an important role in remodeling the extracellular matrix in normal and pathological processes. The effect of phorbol-myristate acetate (PMA), interleukin-1 (IL-1), and tumor necrosis factor-alpha (TNF-alpha) on MMP-1 and TIMP-1 expression was studied on highly purified thyrocytes and undifferentiated 8505 C, C 643, HTh 74, SW 1736 thyroid carcinoma cells compared with thyroid-derived fibroblasts. Messenger RNA (mRNA) levels were monitored by competitive semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) after 24 hours. Culture supernatants were assayed for free and/or complexed MMP-1 and TIMP-1 after 48 hours using enzyme-linked immunosorbent assay (ELISA) systems (detection limit: <2 ng/mL). MMP-1 and TIMP-1 mRNA were present in all cell types, although thyrocytes showed MMP-1 mRNA levels near the detection limit. 8505 C expressed MMP-1 mRNA levels of up to 10(6) times those of the other cells analyzed. PMA and IL-1 increased MMP-1 mRNA in most cell types. TIMP-1 mRNA increased after treatment with PMA in all cells except 8505 C, whereas only slight effects were shown after IL-1 stimulation. MMP-1 protein was undetectable in normal thyrocyte cultures, but was secreted spontaneously by all cell lines ([ng/mL]; C 643: 15+/-7; HTh 74: 81+/-1; SW 1736: 13+/-2; 8505 C: 2097+/-320). There was a strong correlation between levels of MMP-1 mRNA and protein (r = 0.99, p < .0001). PMA and IL-1 increased MMP-1 secretion in all cell types after 48 hours. Fibroblasts ([ng/mL] 517+/-55) and the cell lines (C 643: 142+/-48; HTh 74: 115+/-13; SW 1736: 202+/-14; 8505C: 120+/-19) secreted TIMP-1 in unstimulated cultures, whereas only a trace amount was detected in thyrocyte cultures, even after PMA treatment. IL-1 upregulated TIMP-1 secretion after 48 hours in SW 1736, HTh 74, and C 643 cells. Our data suggest that in contrast to normal thyrocytes, dedifferentiated thyroid carcinoma cell lines are potential producers of MMP-1 as well as TIMP-1. High MMP-1 or MMP-1/TIMP-1 expression may play a role in tissue invasion of undifferentiated thyroid cancer cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1050-7256
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
713-24
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9349574-Adult, pubmed-meshheading:9349574-Carcinoma, pubmed-meshheading:9349574-Collagenases, pubmed-meshheading:9349574-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:9349574-Gene Expression Regulation, Enzymologic, pubmed-meshheading:9349574-Gene Expression Regulation, Neoplastic, pubmed-meshheading:9349574-Humans, pubmed-meshheading:9349574-Interferon-gamma, pubmed-meshheading:9349574-Interleukin-1, pubmed-meshheading:9349574-Matrix Metalloproteinase 1, pubmed-meshheading:9349574-Middle Aged, pubmed-meshheading:9349574-Polymerase Chain Reaction, pubmed-meshheading:9349574-RNA, Messenger, pubmed-meshheading:9349574-RNA, Neoplasm, pubmed-meshheading:9349574-Receptors, Cytokine, pubmed-meshheading:9349574-Tetradecanoylphorbol Acetate, pubmed-meshheading:9349574-Thyroid Gland, pubmed-meshheading:9349574-Thyroid Neoplasms, pubmed-meshheading:9349574-Tissue Inhibitor of Metalloproteinase-1, pubmed-meshheading:9349574-Tumor Cells, Cultured, pubmed-meshheading:9349574-Tumor Necrosis Factor-alpha
pubmed:year
1997
pubmed:articleTitle
Human thyroid carcinoma cell lines and normal thyrocytes: expression and regulation of matrix metalloproteinase-1 and tissue matrix metalloproteinase inhibitor-1 messenger-RNA and protein.
pubmed:affiliation
Institut of Anatomy, University of Leipzig, Germany.
pubmed:publicationType
Journal Article, Comparative Study