Linked Life Data

8858003

Source:http://linkedlifedata.com/resource/pubmed/id/8858003

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1996-11-19
pubmed:abstractText
To identify potential molecular substrates for leukocyte trafficking and activation in multiple sclerosis (MS) brain, we determined the immunocytochemical distribution of the beta, integrin lymphocyte-function-associated antigen-1 (LFA-1) and its major ligands, intercellular adhesion molecule (ICAM)-1, ICAM-2, and ICAM-3 in MS tissue. Colocalization of these adhesion molecules with lineage-specific markers was analyzed by dual-labeling immunocytochemistry and confocal microscopy. ICAM-1 and ICAM-2 were detected on endothelial cells, and ICAM-3 immunoreactivity was restricted to infiltrating leukocytes. In control brain, 10% of glucose transporter-1 positive vessels contained ICAM-1 immunoreactivity on their luminal surface and 21% were ICAM-2-positive. A significant increase in ICAM-1-positive vessels was found in MS brains. This increase was greater in MS lesions (81% of vessels) than in nonlesion areas (37% of vessels). A significant increase in ICAM-1-positive vessels was found in encephalitis (55% of vessels) but not in Parkinson's (17% of vessels) brains. The percentage of vessels expressing ICAM-2 was not increased in MS, encephalitis, or Parkinson's brains. Both ICAM-3 and LFA-1 were detected on the vast majority of infiltrating lymphocytes and monocytes in and near MS lesions, and these cells were often closely apposed to each other. In addition, LFA-1 was detected on activated microglia located close to the edge of demyelinating lesions. ICAM-3-positive leukocytes were often closely apposed to LFA-1-positive microglia. These results suggest a role for ICAM-1, -2, and LFA-1 in the transendothelial migration of leukocytes into MS brain and a role for ICAM 3/LFA-1 interactions in the activation of lymphocytes, monocytes, and microglia in MS lesions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-3069
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1060-72
pubmed:dateRevised
2008-6-13
pubmed:meshHeading
pubmed-meshheading:8858003-Adolescent, pubmed-meshheading:8858003-Adult, pubmed-meshheading:8858003-Aged, pubmed-meshheading:8858003-Aged, 80 and over, pubmed-meshheading:8858003-Antigens, CD, pubmed-meshheading:8858003-Antigens, Differentiation, pubmed-meshheading:8858003-Brain, pubmed-meshheading:8858003-Brain Chemistry, pubmed-meshheading:8858003-Cell Adhesion Molecules, pubmed-meshheading:8858003-Child, pubmed-meshheading:8858003-Child, Preschool, pubmed-meshheading:8858003-Endothelium, pubmed-meshheading:8858003-Humans, pubmed-meshheading:8858003-Infant, pubmed-meshheading:8858003-Infant, Newborn, pubmed-meshheading:8858003-Intercellular Adhesion Molecule-1, pubmed-meshheading:8858003-Leukocytes, pubmed-meshheading:8858003-Lymphocyte Function-Associated Antigen-1, pubmed-meshheading:8858003-Microscopy, Confocal, pubmed-meshheading:8858003-Middle Aged, pubmed-meshheading:8858003-Multiple Sclerosis
pubmed:year
1996
pubmed:articleTitle
Distribution of immunoglobulin superfamily members ICAM-1, -2, -3, and the beta 2 integrin LFA-1 in multiple sclerosis lesions.
pubmed:affiliation
Department of Neurosciences, Cleveland Clinic Foundation, Ohio 44195, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't