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rdf:type |
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lifeskim:mentions |
umls-concept:C0013138,
umls-concept:C0033684,
umls-concept:C0076919,
umls-concept:C0086418,
umls-concept:C0205245,
umls-concept:C0243037,
umls-concept:C0851285,
umls-concept:C1148673,
umls-concept:C1334043,
umls-concept:C1704222,
umls-concept:C1711351
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pubmed:issue |
6
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pubmed:dateCreated |
1993-7-2
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pubmed:databankReference |
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pubmed:abstractText |
A Drosophila cDNA encoding the largest TFIID subunit (p230) was isolated using a degenerate oligodeoxynucleotide probe based on an amino acid sequence of the purified protein. The entire cDNA sequence contains an open reading frame encoding a polypeptide of 2068 amino acids, corresponding to a calculated molecular mass of 232 kD. The deduced amino acid sequence showed a strong sequence similarity with the protein encoded by a human gene (CCG1) implicated in cell cycle progression through G1, suggesting that p230 may be a target for cell cycle regulatory factors. The recombinant protein expressed in Sf9 cells via a baculovirus vector interacts directly with the TATA box-binding subunit of TFIID (TFIID tau or TBP) from Drosophila, human, and yeast. Surprisingly, recombinant p230 inhibits the TATA box-binding activity and function of TFIID tau, suggesting that p230 interactions with TFIID tau, and possible modulations thereof by other factors may play an important role in TFIID function.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1033-46
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8504928-Amino Acid Sequence,
pubmed-meshheading:8504928-Animals,
pubmed-meshheading:8504928-Base Sequence,
pubmed-meshheading:8504928-Cell Cycle,
pubmed-meshheading:8504928-Chromatography, Affinity,
pubmed-meshheading:8504928-Cloning, Molecular,
pubmed-meshheading:8504928-DNA,
pubmed-meshheading:8504928-DNA-Binding Proteins,
pubmed-meshheading:8504928-Drosophila,
pubmed-meshheading:8504928-G1 Phase,
pubmed-meshheading:8504928-High Mobility Group Proteins,
pubmed-meshheading:8504928-Humans,
pubmed-meshheading:8504928-Molecular Sequence Data,
pubmed-meshheading:8504928-Open Reading Frames,
pubmed-meshheading:8504928-Peptide Fragments,
pubmed-meshheading:8504928-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8504928-Sequence Homology, Amino Acid,
pubmed-meshheading:8504928-TATA Box,
pubmed-meshheading:8504928-TATA-Box Binding Protein,
pubmed-meshheading:8504928-Transcription Factor TFIID,
pubmed-meshheading:8504928-Transcription Factors
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pubmed:year |
1993
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pubmed:articleTitle |
Drosophila 230-kD TFIID subunit, a functional homolog of the human cell cycle gene product, negatively regulates DNA binding of the TATA box-binding subunit of TFIID.
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pubmed:affiliation |
National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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