Linked Life Data

8405184

Source:http://linkedlifedata.com/resource/pubmed/id/8405184

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1993-10-27
pubmed:abstractText
Drosophila promoter fusion stocks containing a chloramphenicol acetyltransferase (CAT) reporter gene fused to a 2.8 kb DNA fragment from the Rh1 opsin promoter were carotenoid deprived from egg to adult, and then adults were replaced by feeding carrot juice. CAT activity, determined by radiometric assay, was low in deprived flies; it increased rapidly during the first 3 days of replacement and then declined back to the control level. Retinoic acid increased peak CAT activity as much as carrot juice and more than beta-carotene, all-trans retinol or all-trans retinal. These findings suggest that vitamin A serves not only as rhodopsin's chromophore but also influences Rh1 opsin gene transcription. Three stocks with various deletions in the Rh1 opsin promoter lacked the carrot juice-dependent elevation of CAT activity. All three deletions include the region from -701 to -488, suggesting that this region may contain a vitamin A-responsive DNA sequence.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-87
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Increased expression of chloramphenicol acetyltransferase by carotenoid and retinoid replacement in Drosophila opsin promoter fusion stocks.
pubmed:affiliation
Division of Biological Sciences, University of Missouri, Columbia 65211.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.