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rdf:type |
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lifeskim:mentions |
umls-concept:C0005456,
umls-concept:C0017963,
umls-concept:C0021701,
umls-concept:C0063695,
umls-concept:C0079717,
umls-concept:C1321758,
umls-concept:C1514562,
umls-concept:C1549781,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
8
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pubmed:dateCreated |
1994-6-2
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pubmed:abstractText |
In order to identify a binding site for ligand intercellular adhesion molecule-1 (ICAM-1) on the beta 2 integrin lymphocyte function-associated antigen-1 (LFA-1), protein fragments of LFA-1 were made by in vitro translation of a series of constructs which featured domain-sized deletions starting from the N-terminus of the alpha subunit of LFA-1. Monoclonal antibodies and ICAM-1 were tested for their ability to bind to these protein fragments. Results show that the putative divalent cation binding domains V and VI contain an ICAM-1 binding site. A series of consecutive peptides covering these domains indicated two discontinuous areas as specific contact sites: residues 458-467 in domain V and residues 497-516 in domain VI. A three-dimensional model of these domains of LFA-1 was constructed based on the sequence similarity to known EF hands. The two regions critical for the interaction of LFA-1 with ICAM-1 lie adjacent to each other, the first next to the non-functional EF hand in domain V and the second coinciding with the potential divalent cation binding loop in domain VI. The binding of ICAM-1 with the domain V and VI region in solution was not sensitive to divalent cation chelation. In short, a critical motif for ICAM-1 binding to the alpha subunit of LFA-1 is shared between two regions of domains V and VI.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1111094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1252434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1322124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1346139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1349017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1370656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1446685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1555235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1683632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1688848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1695194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1711032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1730629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1970514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1974032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-1985922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2002847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2010917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2015901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2077034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2199576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2303453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2334704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2380249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2479549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2537322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2545729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-2743332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-30693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3145979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3210231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3244696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3313277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3315233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3327687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3340213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3349522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-3600760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-6339253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-6350038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-7678422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-7680657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-7903240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-8458080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-8479524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7909511-875032
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1790-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7909511-Humans,
pubmed-meshheading:7909511-Peptide Fragments,
pubmed-meshheading:7909511-Models, Molecular,
pubmed-meshheading:7909511-Base Sequence,
pubmed-meshheading:7909511-Amino Acid Sequence,
pubmed-meshheading:7909511-Protein Binding,
pubmed-meshheading:7909511-Binding Sites,
pubmed-meshheading:7909511-Dose-Response Relationship, Drug,
pubmed-meshheading:7909511-Molecular Sequence Data,
pubmed-meshheading:7909511-Binding, Competitive,
pubmed-meshheading:7909511-Structure-Activity Relationship,
pubmed-meshheading:7909511-T-Lymphocytes,
pubmed-meshheading:7909511-Cations, Divalent,
pubmed-meshheading:7909511-Sequence Homology, Amino Acid,
pubmed-meshheading:7909511-Antibodies, Monoclonal
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