Linked Life Data

20030941

Source:http://linkedlifedata.com/resource/pubmed/id/20030941

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-12-24
pubmed:abstractText
This study was aimed to investigate the activity of matrix metalloproteinase-9 (MMP-9) in umbilical cord blood mononuclear cells, the effect of recombinant human tumor necrosis factor alpha (rhTNF-alpha) on the activity of MMP-9 and cell migration capability of umbilical cord blood mononuclear cells, as well as the relationship between them. The different concentrations of TNF-alpha was added into the mononuclear cells. The activity of MMP-9 in umbilical cord blood mononuclear cells were analyzed by gelatinase spectrum. The cell migration capability was observed through the millicell carbine. The results showed that after umbilical cord blood mononuclear cells were cultured in serum-free RPMI 1640 medium for 24 and 48 hours, the activity of MMP-9 could be detected, the cell migration capability at 24 and 48 hours were (1.371 +/- 0.011)% and (1.384 +/- 0.014)% respectively, there was no statistical difference between them. Large dose of TNF-alpha (500 U/ml) led to the death of the cells; small doses of TNF-alpha (< 200 U/ml) up-regulated the activity of MMP-9, and as the concentration of TNF-alpha increased, the activity of MMP-9 was enhanced. Small doses of TNF-alpha (< 200 U/ml) improved the migration capability of umbilical cord blood mononuclear cells in a dose-dependent manner. It is concluded that small doses of TNF-alpha can upregulate the activity, enhance the migration capability of umbilical cord blood mononuclear cells, which probably accelerates cell homing after umbilical cord blood stem cell transplant and improves the hematopoietic reconstruction after transplantation.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1009-2137
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1532-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
[Effect of recombinant human tumor necrosis factor alpha on activity of gelatinase B and migration of human umbilical cord blood mononuclear cells in vitro].
pubmed:affiliation
Department of Hematology, The First Hospital, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't