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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1990-7-18
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pubmed:abstractText |
Functional studies demonstrate that T cell activation often requires not only occupancy of the TCR but costimulatory interactions of other molecules, which remain largely undefined. We have tested the hypothesis that LFA-1 interaction with its ligand intercellular adhesion molecule 1 (CD54) (ICAM-1) is such a costimulatory interaction in a model system using biochemically purified ICAM-1 and TCR cross-linking by anti-CD3 mAb OKT3 immobilized on plastic. Resting T cells do not respond to OKT3 mAb immobilized on plastic. However ICAM-1 deposited on plastic together with the nonmitogenic immobilized OKT3 results in a potent activating stimulus. This costimulation cannot be readily accounted for by ICAM-1-mediated adhesion but is consistent with a role in signaling, which is observed in ICAM-1-mediated augmentation of activation induced by PMA/ionomycin. The ability of ICAM-1 to costimulate with immobilized CD3 contrasts with minimal costimulatory activity of cytokines IL-1 beta, IL-2, and IL-6. The proliferative response to co-immobilized OKT3 and ICAM-1 is dependent on the IL-2R, which is induced only in the presence of both OKT3 and ICAM-1. The present data demonstrate that LFA-1/ICAM-1 interaction is a potent costimulus for TCR-mediated activation; this observation, interpreted in light of previous reports, suggests that LFA-1/ICAM-1 is of major physiologic importance as a costimulatory signal.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Leukocyte-Adhesion,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
144
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4579-86
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1972160-Antigens, CD3,
pubmed-meshheading:1972160-Antigens, Differentiation,
pubmed-meshheading:1972160-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:1972160-Cell Adhesion,
pubmed-meshheading:1972160-Cell Adhesion Molecules,
pubmed-meshheading:1972160-Cells, Cultured,
pubmed-meshheading:1972160-Humans,
pubmed-meshheading:1972160-Intercellular Adhesion Molecule-1,
pubmed-meshheading:1972160-Interleukin-1,
pubmed-meshheading:1972160-Interleukin-6,
pubmed-meshheading:1972160-Ionomycin,
pubmed-meshheading:1972160-Lymphocyte Activation,
pubmed-meshheading:1972160-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:1972160-Receptors, Antigen, T-Cell,
pubmed-meshheading:1972160-Receptors, Interleukin-2,
pubmed-meshheading:1972160-Receptors, Leukocyte-Adhesion,
pubmed-meshheading:1972160-Signal Transduction,
pubmed-meshheading:1972160-T-Lymphocytes,
pubmed-meshheading:1972160-Tetradecanoylphorbol Acetate
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pubmed:year |
1990
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pubmed:articleTitle |
The LFA-1 ligand ICAM-1 provides an important costimulatory signal for T cell receptor-mediated activation of resting T cells.
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pubmed:affiliation |
Experimental immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article,
In Vitro
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