Source:http://linkedlifedata.com/resource/pubmed/id/19701612
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2010-4-2
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pubmed:abstractText |
The optimization of process parameters for high amylase production by Saccharomycopsis fibuligera A11 in solid-state fermentation was carried out using central composite design. Finally, the optimal parameters obtained with the response surface methodology (RSM) were moisture 610.0 ml/kg, inoculum 30.0 ml (OD(600 nm) = 20.0)/kg, the amount ratio of wheat bran to rice husk 0.42, cassava starch concentration 20.0 g/kg, temperature 28 degrees C, and natural pH. Under the optimized conditions, 4,296 U/g of dry substrate of amylase activity was reached in the solid-state fermentation culture of the yeast strain A11 within 160 h, whereas the predicted maximum amylase activity of 4,222 U/g of dry substrate of amylase activity was derived from the RSM regression. It was found that cassava starch can be actively converted into monosaccharides and oligosaccharides by the crude amylase.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1559-0291
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
252-63
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pubmed:meshHeading |
pubmed-meshheading:19701612-Amylases,
pubmed-meshheading:19701612-Analysis of Variance,
pubmed-meshheading:19701612-Fermentation,
pubmed-meshheading:19701612-Hydrolysis,
pubmed-meshheading:19701612-Manihot,
pubmed-meshheading:19701612-Reproducibility of Results,
pubmed-meshheading:19701612-Saccharomyces,
pubmed-meshheading:19701612-Starch
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pubmed:year |
2010
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pubmed:articleTitle |
Amylase production by Saccharomycopsis fibuligera A11 in solid-state fermentation for hydrolysis of Cassava starch.
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pubmed:affiliation |
Key Laboratory of Marine Genetics and Gene Resource Exploitation (Ministry of Education), Ocean University of China, Yushan Road, No.5, Qingdao, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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