Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-7-2
pubmed:abstractText
Neuropeptide Y (NPY) functions as a peptide neurotransmitter and as a neuroendocrine hormone. The active NPY peptide is generated in secretory vesicles by proteolytic processing of proNPY. Novel findings from this study show that cathepsin L participates as a key proteolytic enzyme for NPY production in secretory vesicles. Notably, NPY levels in cathepsin L knockout (KO) mice were substantially reduced in brain and adrenal medulla by 80% and 90%, respectively. Participation of cathepsin L in producing NPY predicts their colocalization in secretory vesicles, a primary site of NPY production. Indeed, cathepsin L was colocalized with NPY in brain cortical neurons and in chromaffin cells of adrenal medulla, demonstrated by immunofluorescence confocal microscopy. Immunoelectron microscopy confirmed the localization of cathepsin L with NPY in regulated secretory vesicles of chromaffin cells. Functional studies showed that coexpression of proNPY with cathepsin L in neuroendocrine PC12 cells resulted in increased production of NPY. Furthermore, in vitro processing indicated cathepsin L processing of proNPY at paired basic residues. These findings demonstrate a role for cathepsin L in the production of NPY from its proNPY precursor. These studies illustrate the novel biological role of cathepsin L in the production of NPY, a peptide neurotransmitter, and neuroendocrine hormone.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-01A10003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-01A19004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-020003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-029004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-030003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-039004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-040003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-049004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-04S10003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-04S19004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-050003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-059004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-06A10003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-06A19004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-070003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-079004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-080003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-089004, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-090003, http://linkedlifedata.com/resource/pubmed/grant/P01 HL058120-099004
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-10360939, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-10657510, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-11023992, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-11487609, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-11517926, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-12145326, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-12553719, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-12859669, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-12860111, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-12869695, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-14576186, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-15548418, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-1575450, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-15779921, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-16903874, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-17241125, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-17244622, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-2053188, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-2834371, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-2897826, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-6434344, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-7713926, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-8137939, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-8558265, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-8638918, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-9103509, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-9192619, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-9405066, http://linkedlifedata.com/resource/pubmed/commentcorrection/18410501-9422358
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1471-4159
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
384-91
pubmed:dateRevised
2011-9-26
pubmed:meshHeading
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