Linked Life Data

16921490

Source:http://linkedlifedata.com/resource/pubmed/id/16921490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-1-2
pubmed:abstractText
Benzo[alpha]pyrene-7,8-diol-9,10-epoxide (B[a]PDE), the major metabolite of benzo[a]pyrene (B[a]P), shows an ultimate complete carcinogen in various animals and is a causative agent for human cancers. However, its effects on the activation of signal pathways and the expression of genes involved in its carcinogenic effect remain largely unknown. In this study, the effects of B[a]PDE on induction of cyclooxygenase (COX)-2 and the signal pathways leading to the induction were investigated. Treatment of mouse epidermal Cl41 cells with B[a]PDE caused an increase in the expression of COX-2 at both transcription and protein levels, while its parental compound B[a]P did not show significant inductive effect. The COX-2 induction by B[a]PDE was dependent on the activation of mitogen-activated protein kinases (MAPK)s/activation protein (AP)-1 pathway, because inhibition of AP-1 by either overexpression of TAM67 (dominant negative mutant of c-jun), or pretreatment of cells with PD98059 (MEK1/2-ERKs pathway inhibitor) or SB202190 (p38K inhibitor), markedly inhibited B[a]PDE-induced COX-2 expression. In addition, impairment of NF-kappaB pathway by either NEMO-BDBP (an NF-kappaB specific inhibitor) or IkappaB kinase (IKK)beta-KM (dominant negative mutant of IKKbeta) also caused marked reduction of COX-2 induction by B[a]PDE. In contrast, inhibition of nuclear factor of activated T cells (NFAT) with FK506, did not show any effect on B[a]PDE-induced COX-2 expression. Collectively, these data indicate that exposure of Cl41 cells to B[a]PDE can induce COX-2 expression by increasing its transcription, which requires the activation of MAPKs/AP-1 and IKKbeta/NF-kappaB pathways, but not NFAT pathway. In view of the importance of COX-2 in carcinogenesis, we anticipate that the induction of COX-2 by B[a]PDE may coordinate its mutagenic effects to facilitate the development of skin cancer.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0899-1987
pubmed:author
pubmed:copyrightInfo
Copyright 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
32-41
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16921490-7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, pubmed-meshheading:16921490-Animals, pubmed-meshheading:16921490-Blotting, Western, pubmed-meshheading:16921490-Carcinogens, pubmed-meshheading:16921490-Cells, Cultured, pubmed-meshheading:16921490-Cyclooxygenase 2, pubmed-meshheading:16921490-Epidermis, pubmed-meshheading:16921490-Humans, pubmed-meshheading:16921490-I-kappa B Kinase, pubmed-meshheading:16921490-Membrane Proteins, pubmed-meshheading:16921490-Mice, pubmed-meshheading:16921490-Mitogen-Activated Protein Kinases, pubmed-meshheading:16921490-NF-kappa B, pubmed-meshheading:16921490-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16921490-Signal Transduction, pubmed-meshheading:16921490-Transcription Factor AP-1, pubmed-meshheading:16921490-Up-Regulation
pubmed:year
2007
pubmed:articleTitle
Benzo[a]pyrene diol-epoxide (B[a]PDE) upregulates COX-2 expression through MAPKs/AP-1 and IKKbeta/NF-kappaB in mouse epidermal Cl41 cells.
pubmed:affiliation
Nelson Institute of Environmental Medicine, New York University, School of Medicine, Tuxedo, New York, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural