Linked Life Data

15570619

Source:http://linkedlifedata.com/resource/pubmed/id/15570619

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-12-6
pubmed:abstractText
We originally identified the membrane-bound progesterone receptor (mPR) using a screening for genes differentially expressed in liver of rats exposed to dioxin. Recent findings have suggested a role for the mPR in sperm cells, ovary, and brain; however, its mechanisms of action are largely unknown. In this study, we examined the expression pattern of the mPR in liver of rats exposed to dioxin and identified possible mechanisms of its regulation. We observed that mPR expression was induced by dioxin, but was also dependent on the hormonal responsiveness of the tissue. In particular, in male, but not female liver, dioxin induced the expression of the mPR. However, in control, untreated female liver the level of mPR transcript was higher than in control males. Moreover, in breast cancer cells MCF-7 dioxin induced mPR expression. Promoter studies using the luciferase assay indicated that a fragment of approximately 350 bp of the mPR promoter was able to induce luciferase activity in the presence of dioxin, suggesting that the presumptive XREs sites contained in this mPR promoter region are responsive to dioxin. Analysis of mPR protein level confirmed the results observed at the RNA level, both in rat liver and MCF-7 cells. Taken together, these observations suggest the existence of a novel cross-talk between steroid and aromatic hydrocarbon receptors (AhR), and underline the importance of the mPR as a mediator of physiologic effects of the sex hormones.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1040-452X
pubmed:author
pubmed:copyrightInfo
(c) 2005 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
166-74
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15570619-Amino Acid Sequence, pubmed-meshheading:15570619-Animals, pubmed-meshheading:15570619-Breast Neoplasms, pubmed-meshheading:15570619-Cell Line, Tumor, pubmed-meshheading:15570619-Cytochrome P-450 CYP1A1, pubmed-meshheading:15570619-Dioxins, pubmed-meshheading:15570619-Estrogens, pubmed-meshheading:15570619-Female, pubmed-meshheading:15570619-Genes, Reporter, pubmed-meshheading:15570619-Humans, pubmed-meshheading:15570619-Liver, pubmed-meshheading:15570619-Male, pubmed-meshheading:15570619-Molecular Sequence Data, pubmed-meshheading:15570619-Promoter Regions, Genetic, pubmed-meshheading:15570619-Rats, pubmed-meshheading:15570619-Rats, Sprague-Dawley, pubmed-meshheading:15570619-Receptors, Aryl Hydrocarbon, pubmed-meshheading:15570619-Receptors, Progesterone, pubmed-meshheading:15570619-Receptors, Steroid, pubmed-meshheading:15570619-Response Elements, pubmed-meshheading:15570619-Sex Factors, pubmed-meshheading:15570619-Tetrachlorodibenzodioxin, pubmed-meshheading:15570619-Transcriptional Activation
pubmed:year
2005
pubmed:articleTitle
Transcriptional activation of the membrane-bound progesterone receptor (mPR) by dioxin, in endocrine-responsive tissues.
pubmed:affiliation
Department of Veterinary Sciences and Microbiology, University of Arizona, Tucson, AZ 87524, USA. selmin@u.arizona.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural