Linked Life Data

1539464

Source:http://linkedlifedata.com/resource/pubmed/id/1539464

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-3-30
pubmed:abstractText
Formalin-inactivated respiratory syncytial virus (FI-RSV), a lysate of HEp-2 cells and proteins F, G and N, immunopurified from infected cell cultures, were compared for their ability to prevent infection and to enhance changes in lung cytology associated with RSV challenge. Mice were immunized at three weekly intervals with serial dilutions of the preparations and challenged by the nasal route 1 week after the last injection; their lungs were analysed 4 days later. The concentration of the immunogens was adjusted to test at least a range of 2 to 500 ng of proteins per injection. The dose of FI-RSV used for immunization influenced both the protection against infection and the potentiation of lung histopathology. There was a strong correlation between the lesion scores and the proportion of larger cells recovered in bronchoalveolar lavage fluid. We therefore used cytological changes as an index of lung alterations in further experiments. Glycoproteins F and G but not protein N were protective against challenge infection. Potentiation was observed in mice immunized with minute amounts (2 ng per injection) of F, G or N. HEp-2 cell lysate also caused potentiation but this required greater than 125 ng of proteins per injection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0264-410X
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Comparison of the ability of formalin-inactivated respiratory syncytial virus, immunopurified F, G and N proteins and cell lysate to enhance pulmonary changes in Balb/c mice.
pubmed:affiliation
Pasteur Mérieux Sérums et Vaccins, Marcy l'Etoile, France.
pubmed:publicationType
Journal Article, Comparative Study