Linked Life Data

14764449

Source:http://linkedlifedata.com/resource/pubmed/id/14764449

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-5-10
pubmed:abstractText
Mitogen-activated protein kinase (MAPK) pathways transduce signals from a diverse array of extracellular stimuli. The three primary MAPK-signaling pathways are the extracellular regulated kinases (ERK1/2), p38 MAPK, and c-Jun NH(2)-terminal kinase (JNK). Previous research in our laboratory has shown that COX-2-elaborated prostanoids participate in recovery of mucosal barrier function in ischemic-injured porcine ileum. Because COX-2 expression is regulated in part by MAPKs, we postulated that MAPK pathways would play an integral role in recovery of injured mucosa. Porcine mucosa was subjected to 45 min of ischemia, after which tissues were mounted in Ussing chambers, and transepithelial electrical resistance (TER) was monitored as an index of recovery of barrier function. Treatment of tissues with the p38 MAPK inhibitor SB-203580 (0.1 mM) or the ERK1/2 inhibitor PD-98059 (0.1 mM) abolished recovery. Western blot analysis revealed that SB-203580 inhibited upregulation of COX-2 that was observed in untreated ischemic-injured mucosa, whereas PD-98059 had no effect on COX-2 expression. Inhibition of TER recovery by SB-203580 or PD-98059 was overcome by administration of exogenous prostaglandin E(2) (1 microM). The JNK inhibitor SP-600125 (0.1 mM) significantly increased TER and resulted in COX-2 upregulation. COX-2 expression appears to be positively and negatively regulated by the p38 MAPK and the JNK pathways, respectively. Alternatively, ERK1/2 appear to be involved in COX-2-independent reparative events that remain to be defined.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0193-1857
pubmed:author
pubmed:issnType
Print
pubmed:volume
286
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G906-13
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:14764449-Animals, pubmed-meshheading:14764449-Cyclooxygenase 2, pubmed-meshheading:14764449-Electric Impedance, pubmed-meshheading:14764449-Enzyme Activation, pubmed-meshheading:14764449-Enzyme Inhibitors, pubmed-meshheading:14764449-Female, pubmed-meshheading:14764449-Ileum, pubmed-meshheading:14764449-Intestinal Mucosa, pubmed-meshheading:14764449-Ischemia, pubmed-meshheading:14764449-Isoenzymes, pubmed-meshheading:14764449-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:14764449-Male, pubmed-meshheading:14764449-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:14764449-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:14764449-Mitogen-Activated Protein Kinases, pubmed-meshheading:14764449-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:14764449-Recovery of Function, pubmed-meshheading:14764449-Swine, pubmed-meshheading:14764449-p38 Mitogen-Activated Protein Kinases
pubmed:year
2004
pubmed:articleTitle
Mitogen-activated protein kinases regulate COX-2 and mucosal recovery in ischemic-injured porcine ileum.
pubmed:affiliation
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.