Linked Life Data

1338132

Source:http://linkedlifedata.com/resource/pubmed/id/1338132

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-4-7
pubmed:abstractText
Oocytes of Bufo bufo gargarizans were used as an expression system for analyzing structure of exogenous membrane protein and their functions. Poly (A)+ mRNA isolated from rat brain was injected into Toad (Bufo bufo gargarizans) oocytes (50 ng/oocyte). Rat brain kainic acid and gamma-aminobutyric acid(GABA) receptors expressed by the injected mRNA were integrated in the oocyte membrane 48 h after the injection at 19 degrees C. Membrane currents induced by kainic acid (5 x 10(-5) mol/L) and GABA (10(-4) mol/L) were 294.0 +/- 6.4 nA (n = 5) and 309.5 +/- 4.9 nA (n = 4) respectively. The kainic acid induced current reached its maximum value at about 10(-3) mol/L. Moreover, it was observed that the 36Cl- influx of the oocytes microinjected with mRNA was one-fold more rapid than the control oocytes. These results indicate that the oocytes of Bufo bufo gargarizans as those of Xenopus laevis can be used to express membrane proteins (receptors & transports) to acquire their proper functions from exogenous mRNA.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0371-0874
pubmed:author
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
470-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
[The expression of kainate and GABA receptors in oocytes of Bufo bufo gargarizans].
pubmed:affiliation
Shanghai Institute of Cell Biology, Shanghai Joint Laboratory of Life Science, Academia Sinica.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't