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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1993-1-28
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pubmed:databankReference |
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pubmed:abstractText |
We investigated whether single P element insertional mutagenesis could be used to analyze heterochromatin within the Drosophila minichromosome Dp1187. Forty-five insertions of the P[lacZ,rosy+] element onto Dp1187 (recovered among 7,825 transpositions) were highly clustered. None was recovered in centromeric heterochromatin, but 39 occurred about 40 kb from the distal telomere within a 4.7-kb hotspot containing tandem copies of a novel 1.8-kb repetitive DNA sequence. The DNA within and distal to this region lacked essential genes and displayed several other properties characteristic of heterochromatin. The rosy+ genes within the inserted transposons were inhibited by position-effect variegation, and the subtelomeric region was underrepresented in polytene salivary gland cells. These experiments demonstrated that P elements preferentially transpose into a small subset of heterochromatic sites, providing a versatile method for studying the structure and function of these chromosome regions. This approach revealed that a Drosophila chromosome contains a large region of subtelomeric heterochromatin with specific structural and genetic properties.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-1660427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-1683846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2087785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2122452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2127578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2164887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2300052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2496301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2497050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2548737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2558959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2565198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2570681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2694944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2830671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2840331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2840332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2841109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2992080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-2997619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3013723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3037327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3041211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3080746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3286016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3290654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3327470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3907856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-3924529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-4507727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-6309410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-6309411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-6319027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-6350893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-6411353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-6816533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1334894-98261
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
132
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pubmed:geneSymbol |
Sb,
ry
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
737-53
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1334894-Animals,
pubmed-meshheading:1334894-Base Sequence,
pubmed-meshheading:1334894-Chromosomes,
pubmed-meshheading:1334894-DNA Transposable Elements,
pubmed-meshheading:1334894-Drosophila,
pubmed-meshheading:1334894-Female,
pubmed-meshheading:1334894-Gene Expression,
pubmed-meshheading:1334894-Heterochromatin,
pubmed-meshheading:1334894-Male,
pubmed-meshheading:1334894-Molecular Sequence Data,
pubmed-meshheading:1334894-Mutagenesis, Insertional
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pubmed:year |
1992
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pubmed:articleTitle |
Analysis of subtelomeric heterochromatin in the Drosophila minichromosome Dp1187 by single P element insertional mutagenesis.
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pubmed:affiliation |
Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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