Linked Life Data

12538517

Source:http://linkedlifedata.com/resource/pubmed/id/12538517

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2003-1-22
pubmed:abstractText
Drosophila oocyte differentiation is preceded by the formation of a polarised 16-cell cyst from a single progenitor stem cell as a result of four rounds of asymmetric mitosis followed by incomplete cytokinesis. We show that the Orbit/Mast microtubule-associated protein is required at several stages in the formation of such polarised 16-cell cysts. In wild-type cysts, the Orbit/Mast protein not only associates with the mitotic spindle and its poles, but also with the central spindle (spindle remnant), ring canal and fusome, suggesting it participates in interactions between these structures. In orbit mutants, the stem cells and their associated fusomes are eventually lost as Orbit/Mast protein is depleted. The mitotic spindles of those cystocytes that do divide are either diminutive or monopolar, and do not make contact with the fusome. Moreover, the spindle remnants and ring canals fail to differentiate correctly in such cells and the structure of fusome is compromised. The Orbit/Mast protein thus appears to facilitate multiple interactions of the fusome with mitotic spindles and ring canals. This ensures correct growth of the fusome into a branched asymmetrically distributed organelle that is pre-determinative of 16-cell cyst formation and oocyte fate specification. Finally the Orbit/Mast protein is required during mid-oogenesis for the organisation of the polarised microtubule network inside the 16-cell cyst that ensures oocyte differentiation. The localisation of CLIP-190 to such microtubules and to the fusome is dependent upon Orbit/Mast to which it is complexed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
130
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
901-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12538517-Animals, pubmed-meshheading:12538517-Cell Differentiation, pubmed-meshheading:12538517-Cell Division, pubmed-meshheading:12538517-Cell Polarity, pubmed-meshheading:12538517-Chimera, pubmed-meshheading:12538517-Contractile Proteins, pubmed-meshheading:12538517-Drosophila Proteins, pubmed-meshheading:12538517-Drosophila melanogaster, pubmed-meshheading:12538517-Female, pubmed-meshheading:12538517-Microtubule-Associated Proteins, pubmed-meshheading:12538517-Microtubules, pubmed-meshheading:12538517-Mitotic Spindle Apparatus, pubmed-meshheading:12538517-Mutation, pubmed-meshheading:12538517-Oocytes, pubmed-meshheading:12538517-Oogenesis, pubmed-meshheading:12538517-Ovary, pubmed-meshheading:12538517-Phenotype, pubmed-meshheading:12538517-Stem Cells, pubmed-meshheading:12538517-Tubulin
pubmed:year
2003
pubmed:articleTitle
Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis.
pubmed:affiliation
Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EN, UK.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't