Linked Life Data

12454403

Source:http://linkedlifedata.com/resource/pubmed/id/12454403

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2002-11-27
pubmed:abstractText
Interaction of lymphocyte function-associated antigen-1 (LFA-1) with intercellular adhesive molecule-1 (ICAM-1) is important in a number of cellular events, including inflammation, adhesion, transendothelial migration. The aim of this work was to study comparatively the adhesive interaction between LFA-1 and ICAM-1 by a micropipette technique and a flow chamber method, and also to explore the effects of tumor necrosis factor (TNF-alpha), phytohemagglutinin (PHA), and tetramethylpyrazine (TMP) on this interaction. The adhesion probability (Pa) between a lymphocyte cell line SKW-3 expressing LFA-1 and a red blood cell (RBC) coated with soluble ICAM-1 was approached by the micropipette technique, while the flow chamber allowed to observe the firm adhesion of SKW-3 on human umbilical vein endothelial cells (HUVECs). Experimental results show that PHA stimulation of lymphocytes resulted in significant increases in the adhesion probability (Pa) and in number of firmly adhered lymphocytes to HUVECs, but TMP treatment could significantly inhibit such increases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0006-355X
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
179-87
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12454403-Adult, pubmed-meshheading:12454403-Cell Adhesion, pubmed-meshheading:12454403-Cell Culture Techniques, pubmed-meshheading:12454403-Endothelium, Vascular, pubmed-meshheading:12454403-Erythrocytes, pubmed-meshheading:12454403-Female, pubmed-meshheading:12454403-Flow Cytometry, pubmed-meshheading:12454403-Humans, pubmed-meshheading:12454403-Intercellular Adhesion Molecule-1, pubmed-meshheading:12454403-Lymphocyte Activation, pubmed-meshheading:12454403-Lymphocyte Function-Associated Antigen-1, pubmed-meshheading:12454403-Lymphocytes, pubmed-meshheading:12454403-Male, pubmed-meshheading:12454403-Micromanipulation, pubmed-meshheading:12454403-Middle Aged, pubmed-meshheading:12454403-Phytohemagglutinins, pubmed-meshheading:12454403-Pyrazines, pubmed-meshheading:12454403-Tumor Cells, Cultured, pubmed-meshheading:12454403-Tumor Necrosis Factor-alpha, pubmed-meshheading:12454403-Umbilical Veins
pubmed:year
2003
pubmed:articleTitle
Comparative studies of LFA-1/ICAM-1 interaction by micropipette and flow chamber techniques.
pubmed:affiliation
Cell and Tissue Engineering and Mechanics, LEMTA-UMR 7563 CNRS and IFR111 Bioengineering, Faculté de Médecine, 54500, Vandoeuvre-lès-Nancy, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't