Source:http://linkedlifedata.com/resource/pubmed/id/11355660
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2001-5-17
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| pubmed:abstractText |
Inflammatory cytokines are suspected to contribute to the pathogenesis of bovine pneumonic pasteurellosis (BPP) through neutrophil recruitment, leukocyte activation, and the induction of a broad array of soluble inflammatory mediators. An in vivo experimental model of BPP was used to characterize the pulmonary expression kinetics of tumor necrosis factor alpha (TNFalpha), interleukin-1 beta (IL-1beta), and interleukin-8 (IL-8) genes and proteins during the acute phase of disease development. Cytokine expression in bronchoalveolar lavage (BAL) fluid, BAL cells, and pneumonic lung parenchyma was quantitated by northern blot analysis, enzyme-linked immunosorbent assay (ELISA), and in situ hybridization at 2, 4, 8, 16, and 24 hours after endobronchial inoculation of Pasteurella (Mannheimia) haemolytica. Expression of TNFalpha, IL-1beta, and IL-8 was significantly increased in the airways and lung lesions of infected calves as compared with mock-infected controls. Although kinetic patterns varied, peak levels of cytokine mRNA occured within 8 hours postinfection (PI), and peak cytokine concentrations occurred within 16 hours PI. In all samples, IL-8 was expressed to the greatest extent and TNFalpha was least expressed. Expression of TNFalpha was restricted to alveolar macrophages. Alveolar and interstitial macrophages produced IL-1beta and IL-8 in the first 4 hours; bronchial and bronchiolar epithelial cells were also significant sources of IL-8 during this period. By 8 hours PI, neutrophils were the dominant source of both IL-1beta and IL-8. These findings demonstrate a spatial and temporal association between pulmonary expression of inflammatory cytokines and acute lung pathology, supporting the hypothesis that cytokines contribute to inflammatory lung injury in BPP.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0300-9858
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
38
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
297-310
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11355660-Animals,
pubmed-meshheading:11355660-Blotting, Northern,
pubmed-meshheading:11355660-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:11355660-Cattle,
pubmed-meshheading:11355660-Cattle Diseases,
pubmed-meshheading:11355660-Cytokines,
pubmed-meshheading:11355660-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:11355660-Image Processing, Computer-Assisted,
pubmed-meshheading:11355660-In Situ Hybridization,
pubmed-meshheading:11355660-Interleukin-1,
pubmed-meshheading:11355660-Interleukin-8,
pubmed-meshheading:11355660-Lung,
pubmed-meshheading:11355660-Male,
pubmed-meshheading:11355660-Mannheimia haemolytica,
pubmed-meshheading:11355660-Pasteurellosis, Pneumonic,
pubmed-meshheading:11355660-RNA, Messenger,
pubmed-meshheading:11355660-Statistics, Nonparametric,
pubmed-meshheading:11355660-Tumor Necrosis Factor-alpha
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| pubmed:year |
2001
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| pubmed:articleTitle |
Pulmonary expression of tumor necrosis factor alpha, interleukin-1 beta, and interleukin-8 in the acute phase of bovine pneumonic pasteurellosis.
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| pubmed:affiliation |
Department of Clinical and Population Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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