Linked Life Data

11113473

Source:http://linkedlifedata.com/resource/pubmed/id/11113473

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2001-1-23
pubmed:abstractText
Loss of heterozygosity (LOH) of the wild-type allele by structural chromosome aberrations (SCAs), homologous mitotic recombination (HMR) or intra-chromosomal (deletion/amplification) recombination (ICR) plays a crucial role in multistage carcinogenesis. We describe here an in vivo system, enabling the detection of all three chromosome breakage-related events in the same genetic experiment, with eye tissue of Drosophila as targets. This modification of the white/white(+) system enables to measure, simultaneously, HMR and ICR on the X-chromosome, and loss of a ring-shaped X-chromosome, utilizing the eye color gene white. Optimal conditions for the detection and quantification of SCAs (ring-X loss) compared to HMR are discussed in detail. Emerging new techniques comprise the parallel detection of HMR on chromosomes X and 3, using the tumor suppressor gene warts in addition to the X-linked marker white. Another modification of the white/white(+) system measures, again in parallel, HMR and chromosome duplication (non-disjunction).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
455
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Parallel monitoring of mitotic recombination, clastogenicity and teratogenic effects in eye tissue of Drosophila.
pubmed:affiliation
Leiden University Medical Centre, Department of Radiation Genetics and Chemical Mutagenesis, MGC Sylvius Laboratories, Wassenaarseweg 72, 2300 RA, Leiden, The Netherlands. vogel@mail.medfac.leidenuniv.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't