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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2000-11-3
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pubmed:abstractText |
We tested the notion that the mucosal adjuvant cholera toxin (CT) could target, in addition to nasal-associated lymphoreticular tissues, the olfactory nerves/epithelium (ON/E) and olfactory bulbs (OBs) when given intranasally. Radiolabeled CT ((125)I-CT) or CT-B subunit ((125)I-CT-B), when given intranasally to mice, entered the ON/E and OB and persisted for 6 days; however, neither molecule was present in nasal-associated lymphoreticular tissues beyond 24 h. This uptake into olfactory regions was monosialoganglioside (GM1) dependent. Intranasal vaccination with (125)I-tetanus toxoid together with unlabeled CT as adjuvant resulted in uptake into the ON/E but not the OB, whereas (125)I-tetanus toxoid alone did not penetrate into the CNS. We conclude that GM1-binding molecules like CT target the ON/E and are retrograde transported to the OB and may promote uptake of vaccine proteins into olfactory neurons. This raises concerns about the role of GM1-binding molecules that target neuronal tissues in mucosal immunity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4778-82
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11045998-Adjuvants, Immunologic,
pubmed-meshheading:11045998-Administration, Intranasal,
pubmed-meshheading:11045998-Animals,
pubmed-meshheading:11045998-Axonal Transport,
pubmed-meshheading:11045998-Brain,
pubmed-meshheading:11045998-Cholera Toxin,
pubmed-meshheading:11045998-Cholera Vaccines,
pubmed-meshheading:11045998-G(M1) Ganglioside,
pubmed-meshheading:11045998-Iodine Radioisotopes,
pubmed-meshheading:11045998-Mice,
pubmed-meshheading:11045998-Mice, Inbred C57BL,
pubmed-meshheading:11045998-Nasal Mucosa,
pubmed-meshheading:11045998-Neurons,
pubmed-meshheading:11045998-Olfactory Bulb,
pubmed-meshheading:11045998-Olfactory Nerve,
pubmed-meshheading:11045998-Organ Specificity,
pubmed-meshheading:11045998-Peptide Fragments,
pubmed-meshheading:11045998-Tissue Distribution
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pubmed:year |
2000
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pubmed:articleTitle |
Cutting edge: the mucosal adjuvant cholera toxin redirects vaccine proteins into olfactory tissues.
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pubmed:affiliation |
Department of Microbiology, Immunobiology Vaccine Center, University of Alabama, Birmingham, AL 35294, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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