Linked Life Data

10810313

Source:http://linkedlifedata.com/resource/pubmed/id/10810313

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-6-21
pubmed:abstractText
Oestrogen stimulates the proliferation of pituitary cells. The present study was designed to clarify the involvement of transforming growth factor-alpha (TGF-alpha) in the oestrogen-induced growth of mouse pituitary cells in vitro. Anterior pituitary cells obtained from ICR male mice were cultured in a primary serum-free culture system. Proliferation of pituitary cells was detected by monitoring the cellular uptake of bromodeoxyuridine. Secretory cell types were immunocytochemically determined. Treatment with TGF-alpha (0.1 and 1 ng/ml) for 5 days stimulated cell proliferation. Since TGF-alpha binds to the epidermal growth factor (EGF) receptor, this action may be exerted through the EGF receptor. Oestradiol-17beta (OE(2), 10(-)(9) M) stimulated mammotrophic and corticotrophic cell proliferation. RG-13022, an EGF receptor inhibitor, inhibited the cell proliferation induced by EGF or OE(2), showing that the EGF receptor was involved in the growth response in mammotrophs and corticotrophs. Treatment with antisense TGF-alpha oligodeoxynucleotide (ODN) inhibited the cell proliferation induced by OE(2), but treatment with antisense EGF ODN did not. RT-PCR analysis revealed that OE(2) stimulated TGF-alpha mRNA and EGF receptor mRNA expression. These results indicate that TGF-alpha mediates the stimulatory effect of oestrogen on the pituitary cell proliferation in a paracrine or autocrine manner, and that EGF receptor expression is stimulated by oestrogen.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-0795
pubmed:author
pubmed:issnType
Print
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
493-501
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10810313-Animals, pubmed-meshheading:10810313-Cell Division, pubmed-meshheading:10810313-Cells, Cultured, pubmed-meshheading:10810313-Epidermal Growth Factor, pubmed-meshheading:10810313-Estradiol, pubmed-meshheading:10810313-Gene Expression, pubmed-meshheading:10810313-Male, pubmed-meshheading:10810313-Mice, pubmed-meshheading:10810313-Mice, Inbred ICR, pubmed-meshheading:10810313-Oligonucleotides, Antisense, pubmed-meshheading:10810313-Pituitary Gland, Anterior, pubmed-meshheading:10810313-RNA, Messenger, pubmed-meshheading:10810313-Receptor, Epidermal Growth Factor, pubmed-meshheading:10810313-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10810313-Stimulation, Chemical, pubmed-meshheading:10810313-Transforming Growth Factor alpha, pubmed-meshheading:10810313-Tyrphostins
pubmed:year
2000
pubmed:articleTitle
Transforming growth factor-alpha stimulates proliferation of mammotrophs and corticotrophs in the mouse pituitary.
pubmed:affiliation
Department of Biology, Faculty of Science, Okayama University, Tsushima, Okayama 700-8530, Japan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't