Source:http://linkedlifedata.com/resource/pubmed/id/10644674
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001721,
umls-concept:C0013138,
umls-concept:C0015306,
umls-concept:C0017337,
umls-concept:C0017973,
umls-concept:C0019143,
umls-concept:C0079427,
umls-concept:C0162610,
umls-concept:C0204514,
umls-concept:C0439849,
umls-concept:C0445223,
umls-concept:C0694878,
umls-concept:C1515655,
umls-concept:C1552599,
umls-concept:C1704787,
umls-concept:C1707871
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pubmed:issue |
4
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pubmed:dateCreated |
2000-2-29
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pubmed:abstractText |
We have devised a sensitive method for the isolation and structural analysis of glycosaminoglycans from two genetically tractable model organisms, the fruit fly, Drosophila melanogaster, and the nematode, Caenorhabditis elegans. We detected chondroitin/chondroitin sulfate- and heparan sulfate-derived disaccharides in both organisms. Chondroitinase digestion of glycosaminoglycans from adult Drosophila produced both nonsulfated and 4-O-sulfated unsaturated disaccharides, whereas only unsulfated forms were detected in C. elegans. Heparin lyases released disaccharides bearing N-, 2-O-, and 6-O-sulfated species, including mono-, di-, and trisulfated forms. We observed tissue- and stage-specific differences in both chondroitin sulfate and heparan sulfate composition in Drosophila. We have also applied these methods toward the analysis of tout-velu, an EXT-related gene in Drosophila that controls the tissue distribution of the growth factor Hedgehog. The proteins encoded by the vertebrate tumor suppressor genes EXT1 and 2, show heparan sulfate co-polymerase activity, and it has been proposed that tout-velu affects Hedgehog activity via its role in heparan sulfate biosynthesis. Analysis of total glycosaminoglycans from tout-velu mutant larvae show marked reductions in heparan sulfate but not chondroitin sulfate, consistent with its proposed function as a heparan sulfate co-polymerase.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2269-75
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10644674-Animals,
pubmed-meshheading:10644674-Caenorhabditis elegans,
pubmed-meshheading:10644674-Carbohydrate Conformation,
pubmed-meshheading:10644674-Cattle,
pubmed-meshheading:10644674-Chromatography, High Pressure Liquid,
pubmed-meshheading:10644674-Drosophila,
pubmed-meshheading:10644674-Drosophila Proteins,
pubmed-meshheading:10644674-Genes, Tumor Suppressor,
pubmed-meshheading:10644674-Heparitin Sulfate,
pubmed-meshheading:10644674-Membrane Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Structural analysis of glycosaminoglycans in Drosophila and Caenorhabditis elegans and demonstration that tout-velu, a Drosophila gene related to EXT tumor suppressors, affects heparan sulfate in vivo.
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pubmed:affiliation |
Department of Molecular Biology, University of Arizona, Tucson, Arizona 85721, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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