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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1999-2-26
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pubmed:abstractText |
We targeted expression of human/fly chimeric Bcr-Abl proteins to the developing central nervous system (CNS) and eye imaginal disc of Drosophila melanogaster. Neural expression of human/fly chimeric P210 Bcr-Abl or P185 Bcr-Abl rescued abl mutant flies from pupal lethality, indicating that P210 and P185 Bcr-Abl can substitute functionally for Drosophila Abl during axonogenesis. However, increased levels of neurally expressed P210 or P185 Bcr-Abl but not Drosophila Abl produced CNS defects and lethality. Expression of P210 or P185 in the eye imaginal disc produced a dominant rough eye phenotype that was dependent on dosage of the transgene. Drosophila Enabled, previously identified as a suppressor of the abl mutant phenotype and substrate for Drosophila Abl kinase, had markedly increased phosphotyrosine levels in Bcr-Abl expressing Drosophila, indicating that it is a substrate for Bcr-Abl as well. Drosophila, therefore, is a suitable model system to identify Bcr-Abl interactions important for signal transduction and oncogenesis.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0950-9232
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
219-32
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9926937-Animals,
pubmed-meshheading:9926937-Animals, Genetically Modified,
pubmed-meshheading:9926937-Central Nervous System,
pubmed-meshheading:9926937-DNA-Binding Proteins,
pubmed-meshheading:9926937-Drosophila melanogaster,
pubmed-meshheading:9926937-Eye,
pubmed-meshheading:9926937-Fusion Proteins, bcr-abl,
pubmed-meshheading:9926937-Gene Expression,
pubmed-meshheading:9926937-Genes, abl,
pubmed-meshheading:9926937-Humans,
pubmed-meshheading:9926937-Morphogenesis,
pubmed-meshheading:9926937-Phosphorylation,
pubmed-meshheading:9926937-Substrate Specificity
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pubmed:year |
1999
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pubmed:articleTitle |
Dominant effects of the bcr-abl oncogene on Drosophila morphogenesis.
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pubmed:affiliation |
Department of Medicine, University of Wisconsin Comprehensive Cancer Center, Madison 53706, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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