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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
22
|
| pubmed:dateCreated |
1998-12-11
|
| pubmed:abstractText |
Light activation of Drosophila photoreceptors leads to the generation of a depolarizing receptor potential via opening of transient receptor potential and transient receptor potential-like cationic channels. Counteracting the light-activated depolarizing current are two voltage-gated K+ conductances, IA and IK, that are expressed in these sensory neurons. Here we show that Drosophila photoreceptors IA and IK are regulated by calcium-calmodulin (Ca2+/calmodulin) via a Ca2+/calmodulin-dependent protein kinase (CaM kinase), with IK being far more sensitive than IA. Inhibition of Ca2+/calmodulin by N-(6 aminohexyl)-5-chloro-1-naphthalenesulfonamide or trifluoperazine markedly reduced the K+ current amplitudes. Likewise, inhibition of CaM kinases by KN-93 potently depressed IK and accelerated its C-type inactivation kinetics. The effect of KN-93 was specific because its structurally related but functionally inactive analog KN-92 was totally ineffective. In Drosophila photoreceptor mutant ShKS133, which allows isolation of IK, we demonstrate by current-clamp recording that inhibition of IK by quinidine or tetraethylammonium increased the amplitude of the photoreceptor potential, depressed light adaptation, and slowed down the termination of the light response. Similar results were obtained when CaM kinases were blocked by KN-93. These findings place photoreceptor K+ channels as an additional target for Ca2+/calmodulin and suggest that IK is well suited to act in concert with other components of the signaling machinery to sharpen light response termination and fine tune photoreceptor sensitivity during light adaptation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzylamines,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/KN 93,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Quinidine,
http://linkedlifedata.com/resource/pubmed/chemical/Shaker Superfamily of Potassium...,
http://linkedlifedata.com/resource/pubmed/chemical/Shaker protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/Tetraethylammonium,
http://linkedlifedata.com/resource/pubmed/chemical/Vasodilator Agents,
http://linkedlifedata.com/resource/pubmed/chemical/W 7
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0270-6474
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
9153-62
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9801355-Adaptation, Ocular,
pubmed-meshheading:9801355-Animals,
pubmed-meshheading:9801355-Benzylamines,
pubmed-meshheading:9801355-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:9801355-Drosophila,
pubmed-meshheading:9801355-Drosophila Proteins,
pubmed-meshheading:9801355-Enzyme Inhibitors,
pubmed-meshheading:9801355-Kinetics,
pubmed-meshheading:9801355-Membrane Potentials,
pubmed-meshheading:9801355-Patch-Clamp Techniques,
pubmed-meshheading:9801355-Photoreceptor Cells, Invertebrate,
pubmed-meshheading:9801355-Potassium Channels,
pubmed-meshheading:9801355-Quinidine,
pubmed-meshheading:9801355-Reaction Time,
pubmed-meshheading:9801355-Shaker Superfamily of Potassium Channels,
pubmed-meshheading:9801355-Sulfonamides,
pubmed-meshheading:9801355-Tetraethylammonium,
pubmed-meshheading:9801355-Vasodilator Agents,
pubmed-meshheading:9801355-Vision, Ocular
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
A Ca2+/calmodulin-dependent protein kinase modulates Drosophila photoreceptor K+ currents: a role in shaping the photoreceptor potential.
|
| pubmed:affiliation |
Neurobiology Department, Weizmann Institute of Science, Rehovot 76100, Israel.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|