Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-7-30
pubmed:abstractText
The intrathecal Ab response to Ag introduced into the normal brain has not been fully explored. Involvement of Ag-specific, peripheral B cells in an intrathecal response was studied using a normal rat model of Ag infusion through an indwelling cannula into defined brain sites, while maintaining a functionally intact blood-brain barrier. Specific Ab was detected in serum and cerebrospinal fluid. The intrathecal response is first detectable at day 14. Isoelectric focusing of cerebrospinal fluid reveals banding patterns consistent with local Ab production. To increase Ag-specific, circulating peripheral lymphocytes available for trafficking to Ag-stimulated brain and for enhancing intrathecal Ab synthesis, rats were preimmunized peripherally. Subsequently, Ag or saline (control) was infused through the cannula. Under this protocol, intrathecal synthesis is detectable earlier (day 5 postinfusion). Immunohistochemical studies at the infusion site assessed Ag-specific B cells, T cells, and activated APCs. Rats receiving peripheral preimmunization followed by Ag into caudate nucleus have far greater numbers of these cells, including plasma cells, within the infusion site compared with saline controls. Results confirm previous indirect evidence of intrathecal Ab synthesis in normal rat brain and provide the first direct evidence for B cell trafficking across normal brain barriers plus retention at the Ag deposition site. Our studies indicate that the normal brain microenvironment supports development of Ag-directed humoral immunity. We propose that immune privilege in normal brain is characterized by down-regulation of cell-mediated but not Ab immune responses within the central nervous system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
161
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
692-701
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9670944-Animals, pubmed-meshheading:9670944-Antigens, pubmed-meshheading:9670944-B-Lymphocyte Subsets, pubmed-meshheading:9670944-Blood-Brain Barrier, pubmed-meshheading:9670944-Brain, pubmed-meshheading:9670944-Brain Chemistry, pubmed-meshheading:9670944-Epitopes, B-Lymphocyte, pubmed-meshheading:9670944-Freund's Adjuvant, pubmed-meshheading:9670944-Humans, pubmed-meshheading:9670944-Immune Sera, pubmed-meshheading:9670944-Immunization Schedule, pubmed-meshheading:9670944-Immunohistochemistry, pubmed-meshheading:9670944-Injections, Intramuscular, pubmed-meshheading:9670944-Injections, Intraventricular, pubmed-meshheading:9670944-Injections, Spinal, pubmed-meshheading:9670944-Male, pubmed-meshheading:9670944-Ovalbumin, pubmed-meshheading:9670944-Rats, pubmed-meshheading:9670944-Rats, Sprague-Dawley, pubmed-meshheading:9670944-Serum Albumin
pubmed:year
1998
pubmed:articleTitle
Antigen-dependent intrathecal antibody synthesis in the normal rat brain: tissue entry and local retention of antigen-specific B cells.
pubmed:affiliation
Division of Biology and Medicine, Brown University, Providence, RI 02912, USA. Paul_Knopf@Brown.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.