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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1998-4-23
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pubmed:abstractText |
"Stress-regulated" mitogen-activated protein kinases (SR-MAPKs) comprise the stress-activated protein kinases (SAPKs)/c-Jun N-terminal kinases (JNKs) and the p38-MAPKs. In the perfused heart, ischemia/reperfusion activates SR-MAPKs. Although the agent(s) directly responsible is unclear, reactive oxygen species are generated during ischemia/reperfusion. We have assessed the ability of oxidative stress (as exemplified by H2O2) to activate SR-MAPKs in the perfused heart and compared it with the effect of ischemia/reperfusion. H2O2 activated both SAPKs/JNKs and p38-MAPK. Maximal activation by H2O2 in both cases was observed at 0.5 mM. Whereas activation of p38-MAPK by H2O2 was comparable to that of ischemia and ischemia/reperfusion, activation of the SAPKs/JNKs was less than that of ischemia/reperfusion. As with ischemia/reperfusion, there was minimal activation of the ERK MAPK subfamily by H2O2. MAPK-activated protein kinase 2 (MAPKAPK2), a downstream substrate of p38-MAPKs, was activated by H2O2 to a similar extent as with ischemia or ischemia/reperfusion. In all instances, activation of MAPKAPK2 in perfused hearts was inhibited by SB203580, an inhibitor of p38-MAPKs. Perfusion of hearts at high aortic pressure (20 kilopascals) also activated the SR-MAPKs and MAPKAPK2. Free radical trapping agents (dimethyl sulfoxide and N-t-butyl-alpha-phenyl nitrone) inhibited the activation of SR-MAPKs and MAPKAPK2 by ischemia/reperfusion. These data are consistent with a role for reactive oxygen species in the activation of SR-MAPKs during ischemia/reperfusion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radical Scavengers,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/MAP-kinase-activated kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/SB 203580,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7228-34
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9516415-Amino Acid Sequence,
pubmed-meshheading:9516415-Animals,
pubmed-meshheading:9516415-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:9516415-Chromatography, High Pressure Liquid,
pubmed-meshheading:9516415-Enzyme Activation,
pubmed-meshheading:9516415-Enzyme Inhibitors,
pubmed-meshheading:9516415-Free Radical Scavengers,
pubmed-meshheading:9516415-Heart,
pubmed-meshheading:9516415-Humans,
pubmed-meshheading:9516415-Hydrogen Peroxide,
pubmed-meshheading:9516415-Imidazoles,
pubmed-meshheading:9516415-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:9516415-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:9516415-Male,
pubmed-meshheading:9516415-Mitogen-Activated Protein Kinases,
pubmed-meshheading:9516415-Molecular Sequence Data,
pubmed-meshheading:9516415-Myocardial Ischemia,
pubmed-meshheading:9516415-Myocardial Reperfusion,
pubmed-meshheading:9516415-Myocardium,
pubmed-meshheading:9516415-Oxidative Stress,
pubmed-meshheading:9516415-Perfusion,
pubmed-meshheading:9516415-Protein-Serine-Threonine Kinases,
pubmed-meshheading:9516415-Pyridines,
pubmed-meshheading:9516415-Rats,
pubmed-meshheading:9516415-Rats, Sprague-Dawley,
pubmed-meshheading:9516415-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
1998
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pubmed:articleTitle |
Stimulation of "stress-regulated" mitogen-activated protein kinases (stress-activated protein kinases/c-Jun N-terminal kinases and p38-mitogen-activated protein kinases) in perfused rat hearts by oxidative and other stresses.
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pubmed:affiliation |
National Heart and Lung Institute Division, Imperial College School of Medicine, Royal Brompton Campus, London SW3 6LY, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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