Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3 Pt 1
|
| pubmed:dateCreated |
1997-10-23
|
| pubmed:abstractText |
We have cloned and expressed recombinant guinea pig tumor necrosis factor-alpha (gpTNF-alpha) and examined its inflammatory activities after tracheal instillation in guinea pigs. A 1,071-bp cDNA, including the region encoding the full-length 234-amino acid gpTNF-alpha protein, was cloned from concanavalin A-stimulated guinea pig splenocytes. The 154-amino acid protein corresponding to secreted gpTNF-alpha was expressed as a fusion protein in Escherichia coli, purified by affinity chromatography, and cleaved to yield a 17-kDa protein. gpTNF-alpha had a cytotoxic effect on WEHI 164 cells and was detected by goat anti-murine tumor necrosis factor-alpha (TNF-alpha) antibody in Western blots. Intratracheal instillation of gpTNF-alpha (50-150 ng) caused pronounced and dose-dependent airway eosinophilia. Incubation of gpTNF-alpha with rabbit anti-murine TNF-alpha sera or heating the gpTNF-alpha before instillation reduced bronchoalveolar lavage (BAL) eosinophils to near control levels. Maximum BAL eosinophilia was observed at 24 h, but eosinophil numbers remained significantly above vehicle-treated animals for 72 h. Hence, gpTNF-alpha elicits a pronounced and protracted eosinophil accumulation in the guinea pig lung.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
273
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
L524-30
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9316485-Amino Acid Sequence,
pubmed-meshheading:9316485-Animals,
pubmed-meshheading:9316485-Antibodies,
pubmed-meshheading:9316485-Base Sequence,
pubmed-meshheading:9316485-Chemotaxis, Leukocyte,
pubmed-meshheading:9316485-Cloning, Molecular,
pubmed-meshheading:9316485-DNA Primers,
pubmed-meshheading:9316485-Eosinophils,
pubmed-meshheading:9316485-Escherichia coli,
pubmed-meshheading:9316485-Female,
pubmed-meshheading:9316485-Guinea Pigs,
pubmed-meshheading:9316485-Humans,
pubmed-meshheading:9316485-Inflammation,
pubmed-meshheading:9316485-Leukocytes,
pubmed-meshheading:9316485-Lung,
pubmed-meshheading:9316485-Male,
pubmed-meshheading:9316485-Mice,
pubmed-meshheading:9316485-Molecular Sequence Data,
pubmed-meshheading:9316485-Polymerase Chain Reaction,
pubmed-meshheading:9316485-Rabbits,
pubmed-meshheading:9316485-Rats,
pubmed-meshheading:9316485-Recombinant Proteins,
pubmed-meshheading:9316485-Sequence Alignment,
pubmed-meshheading:9316485-Sequence Homology, Amino Acid,
pubmed-meshheading:9316485-Spleen,
pubmed-meshheading:9316485-Time Factors,
pubmed-meshheading:9316485-Trachea,
pubmed-meshheading:9316485-Tumor Cells, Cultured,
pubmed-meshheading:9316485-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Airway inflammation induced by recombinant guinea pig tumor necrosis factor-alpha.
|
| pubmed:affiliation |
School of Pharmacy and Pharmacology, University of Bath, United Kingdom.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|