Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1997-7-10
|
| pubmed:abstractText |
The potential of hematopoietic stem cells (HSCs) from human immunodeficiency virus type-1 (HIV-1)-infected individuals, eg, self-renewal and multilineage differentiative capacity, might be perturbed due to the underlying disease. In this study, we assessed the HSC activity in the CD34+ Thy-1+ cell population of peripheral blood stem cells (PBSCs) of three asymptomatic HIV-1-infected individuals after granulocyte colony-stimulating factor (G-CSF; 10 microg/kg/d) mobilization. On day 4 of G-CSF treatment, 0.8% to 1% of the total blood mononuclear cells were CD34+. Leukapheresis followed by a two-step cell isolation process yielded a CD34+ Thy-1+ cell population of high purity (76% to 92% CD34+ Thy-1+ cells). This cell population showed no evidence of HIV-1-containing cells based on a semiquantitative HIV-1 DNA polymerase chain reaction. Furthermore, the purified cells showed normal hematopoietic potential in in vitro clonogenic assays. Successful gene transfer into committed progenitor cells (colony-forming units-cells) and more primitive stem/progenitor cells (long-term culture colony-forming cells) could be shown after amphotropic retroviral transduction. These data provide evidence that the CD34+ Thy-1+ stem cell compartment can be mobilized and enriched in early stage HIV-1-infected patients. Furthermore, successful transduction of this cell population as a prerequisite for stem cell-based clinical gene therapy protocols was demonstrated.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating...,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Core Protein p24
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
89
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4299-306
|
| pubmed:dateRevised |
2006-4-21
|
| pubmed:meshHeading |
pubmed-meshheading:9192752-Adult,
pubmed-meshheading:9192752-Antigens, CD34,
pubmed-meshheading:9192752-Antigens, Thy-1,
pubmed-meshheading:9192752-Blood Cell Count,
pubmed-meshheading:9192752-Cell Separation,
pubmed-meshheading:9192752-DNA, Viral,
pubmed-meshheading:9192752-Feasibility Studies,
pubmed-meshheading:9192752-Female,
pubmed-meshheading:9192752-Flow Cytometry,
pubmed-meshheading:9192752-Genetic Vectors,
pubmed-meshheading:9192752-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:9192752-HIV Core Protein p24,
pubmed-meshheading:9192752-HIV Infections,
pubmed-meshheading:9192752-Hematopoiesis,
pubmed-meshheading:9192752-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:9192752-Hematopoietic Stem Cells,
pubmed-meshheading:9192752-Humans,
pubmed-meshheading:9192752-Leukapheresis,
pubmed-meshheading:9192752-Male,
pubmed-meshheading:9192752-Middle Aged,
pubmed-meshheading:9192752-Polymerase Chain Reaction,
pubmed-meshheading:9192752-Retroviridae,
pubmed-meshheading:9192752-Viremia
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Hematopoietic potential and retroviral transduction of CD34+ Thy-1+ peripheral blood stem cells from asymptomatic human immunodeficiency virus type-1-infected individuals mobilized with granulocyte colony-stimulating factor.
|
| pubmed:affiliation |
Progenesys Program, Systemix Inc, Palo Alto, CA 94304, USA.
|
| pubmed:publicationType |
Journal Article
|