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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-7-29
|
| pubmed:databankReference | |
| pubmed:abstractText |
The Drosophila fat facets (faf) gene is a ubiquitin-specific protease necessary for the normal development of the eye and of the syncytial stage embryo in the fly. Using a gene trap approach in embryonic stem cells we have isolated a murine gene with extensive sequence similarity to the Drosophila faf gene and called it Fam (fat facets in mouse). The putative mouse protein shows colinearity and a high degree of sequence identity to the Drosophila protein over almost its entire length of 2554 amino acids. The two enzymatic sites characteristic of ubiquitin-specific proteases are very highly conserved between mice and Drosophila and this conservation extends to yeast. Fam is expressed in a complex pattern during postimplantation development. In situ hybridisation detected Fam transcripts in the rapidly expanding cell populations of gastrulating and neurulating embryos, in post-mitotic cells of the CNS as well as in the apoptotic regions between the digits, indicating that it is not associated with a single developmental or cellular event. The strong sequence similarity to faf and the developmentally regulated expression pattern suggest that Fam and the ubiquitin pathway may play a role in determining cell fate in mammals, as has been established for Drosophila.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0925-4773
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
63
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
29-38
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9178254-Amino Acid Sequence,
pubmed-meshheading:9178254-Animals,
pubmed-meshheading:9178254-Chromosome Mapping,
pubmed-meshheading:9178254-Cloning, Molecular,
pubmed-meshheading:9178254-Conserved Sequence,
pubmed-meshheading:9178254-Crosses, Genetic,
pubmed-meshheading:9178254-Drosophila,
pubmed-meshheading:9178254-Embryo, Mammalian,
pubmed-meshheading:9178254-Embryo, Nonmammalian,
pubmed-meshheading:9178254-Embryonic and Fetal Development,
pubmed-meshheading:9178254-Endopeptidases,
pubmed-meshheading:9178254-Gene Expression Regulation, Developmental,
pubmed-meshheading:9178254-Mice,
pubmed-meshheading:9178254-Molecular Sequence Data,
pubmed-meshheading:9178254-Recombinant Fusion Proteins,
pubmed-meshheading:9178254-Recombinant Proteins,
pubmed-meshheading:9178254-Sequence Alignment,
pubmed-meshheading:9178254-Sequence Homology, Amino Acid,
pubmed-meshheading:9178254-Stem Cells,
pubmed-meshheading:9178254-Transcription, Genetic,
pubmed-meshheading:9178254-X Chromosome
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Cloning and expression analysis of a novel mouse gene with sequence similarity to the Drosophila fat facets gene.
|
| pubmed:affiliation |
Centre for Molecular and Cellular Biology, University of Queensland, St Lucia, Australia. svwood@cmcb.uq.edu.au
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|