|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0012899,
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0040649,
umls-concept:C0086418,
umls-concept:C0172237,
umls-concept:C0185117,
umls-concept:C0205246,
umls-concept:C0258442,
umls-concept:C1333357,
umls-concept:C1415344,
umls-concept:C1415345,
umls-concept:C1521761,
umls-concept:C1705468,
umls-concept:C1711351,
umls-concept:C1880022,
umls-concept:C1998793,
umls-concept:C2911684
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1997-6-17
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|
pubmed:abstractText |
The human cyclin H, a protein normally associated with the cyclin-dependent kinase cdk7, was overexpressed in Escherichia coli using a T7 RNA polymerase expression system and further purified to apparent homogeneity. The purified recombinant cyclin H is similar to the endogenous one according to the following criteria: molecular weight, microsequencing and mass spectra studies, ability to interact with cdk7, and regulatory kinase activity. The scale-up of cyclin H purification is described.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
1046-5928
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
9
|
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pubmed:owner |
NLM
|
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pubmed:authorsComplete |
Y
|
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pubmed:pagination |
153-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9056480-Affinity Labels,
pubmed-meshheading:9056480-Amino Acid Sequence,
pubmed-meshheading:9056480-Blotting, Western,
pubmed-meshheading:9056480-Cyclin H,
pubmed-meshheading:9056480-Cyclins,
pubmed-meshheading:9056480-Escherichia coli,
pubmed-meshheading:9056480-Genetic Vectors,
pubmed-meshheading:9056480-Humans,
pubmed-meshheading:9056480-Molecular Sequence Data,
pubmed-meshheading:9056480-Protein Binding,
pubmed-meshheading:9056480-Recombinant Proteins,
pubmed-meshheading:9056480-Transcription Factors, TFII
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|
pubmed:year |
1997
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|
pubmed:articleTitle |
Expression in Escherichia coli: purification and characterization of cyclin H, a subunit of the human general transcription/DNA repair factor TFIIH.
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|
pubmed:affiliation |
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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