Linked Life Data

9012516

Source:http://linkedlifedata.com/resource/pubmed/id/9012516

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1997-2-24
pubmed:abstractText
During Drosophila oogenesis, developing germline cysts are spanned by a large cytoplasmic structure called a fusome, containing alpha-spectrin and the adducin-like product of the hu-li tai shao (hts) gene. We found that fusomes contain two additional membrane skeletal proteins: beta-spectrin and ankyrin. hts was shown previously to be required for cyst formation and oocyte differentiation; the role of the fusome itself, however, and the organization and function of its other components, remains unclear. Using the FRT/FLP recombinase system to generate clones of alpha-spectrin-deficient cells in the ovary, we have shown that alpha-spectrin is also required for cyst formation and oocyte differentiation, but that its role in each process is distinct from that of Hts protein. Furthermore, alpha-spectrin is required for these processes in germline cells, but not in the follicle cells that surround each cyst. We have also found that the organization of membrane skeletal proteins is more dependent on alpha-spectrin in the fusome than at the plasma membrane in other cells. Our results suggest that the fusome and its associated membrane skeleton play a central role in regulating the divisions and differentiation of cyst cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3959-68
pubmed:dateRevised
2009-9-28
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
alpha-spectrin is required for germline cell division and differentiation in the Drosophila ovary.
pubmed:affiliation
Howard Hughes Medical Institute, Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't