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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6 Pt 1
|
| pubmed:dateCreated |
1997-1-16
|
| pubmed:abstractText |
Chemoattractant cytokines (chemokines) such as RANTES, are potent chemoattractants for eosinophils, T lymphocytes, and monocyte/macrophages. We examined RANTES mRNA and protein expression in bronchial biopsies and bronchoalveolar lavage (BAL) cells from patients with mild asthma on beta 2-adrenergic agonist therapy only. Using quantitative polymerase chain reaction (PCR), mean RANTES starting cDNA was 110 +/- 18 fg/pg beta-actin in asthmatics compared with 33.7 +/- 11.0 fg/pg in normals (p < 0.003) but there was no significant difference in RANTES mRNA expression in BAL cells between the two groups. Immunohistochemical staining of cryostat sections of bronchial biopsies with an anti-RANTES antibody using peroxidase antiperoxidase method revealed constitutive staining in airway epithelial cells, airway smooth muscle, and subepithelial cells. However, there was no difference in the number of RANTES-staining cells between normal subjects and asthmatics despite significantly increased numbers of CD4+ T-cells, CD68+ macrophages, and MBP+ eosinophils in mucosal biopsies obtained from asthmatics. Double-staining revealed expression of RANTES in a proportion of CD3+ T lymphocytes. Thus, RANTES is constitutively expressed in the airways and RANTES mRNA is elevated in airways of patients with mild asthma, supporting a role for RANTES in normal and asthmatic airways.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1073-449X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
154
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1804-11
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8970374-Actins,
pubmed-meshheading:8970374-Adult,
pubmed-meshheading:8970374-Asthma,
pubmed-meshheading:8970374-Biopsy,
pubmed-meshheading:8970374-Bronchi,
pubmed-meshheading:8970374-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:8970374-CD4-Positive T-Lymphocytes,
pubmed-meshheading:8970374-Cell Count,
pubmed-meshheading:8970374-Chemokine CCL5,
pubmed-meshheading:8970374-DNA, Complementary,
pubmed-meshheading:8970374-Eosinophils,
pubmed-meshheading:8970374-Female,
pubmed-meshheading:8970374-Humans,
pubmed-meshheading:8970374-Immunohistochemistry,
pubmed-meshheading:8970374-Macrophages,
pubmed-meshheading:8970374-Male,
pubmed-meshheading:8970374-Polymerase Chain Reaction,
pubmed-meshheading:8970374-RNA, Messenger,
pubmed-meshheading:8970374-T-Lymphocytes
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Expression of RANTES mRNA and protein in airways of patients with mild asthma.
|
| pubmed:affiliation |
National Heart and Lung Institute, Imperial College of Medicine, London, United Kingdom.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|