Linked Life Data

894145

Source:http://linkedlifedata.com/resource/pubmed/id/894145

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1977-10-20
pubmed:abstractText
A method is described for the transesterification of lipids of mammalian tissues for fatty acid analysis by gas-liquid chromatography (GLC) that eliminates the extraction step of conventional procedures. The method involves the direct reaction of anhydrous HCl-methanol with the lipids in approximately 10 mg of tissue or 0.1 ml of serum after removal of water by reacting it with 2,2'-dimethoxypropane (DMP). Acetone and methanol produced from water in the sample, as well as the excess DMP, are evaporated prior to transesterification in order to eliminate the formation of artifacts from the solvents. The method was demonstrated with rat serum and brain tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
540-3
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Direct transesterification of lipids in mammalian tissue for fatty acid analysis via dehydration with 2,2'-dimethoxypropane.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.