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rdf:type |
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lifeskim:mentions |
umls-concept:C0009015,
umls-concept:C0016030,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0026845,
umls-concept:C0035236,
umls-concept:C0086222,
umls-concept:C0086943,
umls-concept:C1145667,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1519249,
umls-concept:C1521761,
umls-concept:C1704973,
umls-concept:C1705733
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pubmed:issue |
1
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pubmed:dateCreated |
1996-10-31
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U23162,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/L13032,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/L20500,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P15277,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P16989,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P16990,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P16991,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P21573,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P21574,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P22568,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/P27817,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/Q00436,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/Q01761,
http://linkedlifedata.com/resource/pubmed/xref/SWISSPROT/U23162
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pubmed:abstractText |
Rous sarcoma virus (RSV) mainly replicates in avian fibroblasts, and the U3 enhancer region of the long terminal repeats of RSV contains the determinants for its tissue-tropic expression. We describe the cloning and characterization of an avian gene that encodes a protein capable of binding to the enhancer region of Rous sarcoma virus. A PCR-derived probe corresponding to the U3 region of RSV was used to isolate a cDNA clone by screening a chicken cDNA expression library. The cDNA is predicted to encode a polypeptide of 298 amino acids that is homologous to the Y-box (inverted CCAAT) family of DNA-binding transcription factors. This factor, which we refer to as Rous sarcoma virus enhancer factor-II (RSV-EF-II), shows 99% aa identity over a 105-amino-acid stretch that is highly conserved in all Y-box proteins, and is commonly referred to as the cold shock domain. RSV-EF-II selectively binds to single-stranded DNA, and the binding site, as determined by electrophoretic mobility shift assays, consists of the sequence 5' GTACCACC 3' located between nucleotides -112 to -119 in the noncoding strand of the RSV enhancer. Although RSV-EF-II shares considerable homology with the Y-box family of proteins, it does not bind to the inverted CCAAT boxes at positions -65 to -69 and -129 to -133 in the RSV LTR. Northern analysis indicates that RSV-EF-II-specific transcripts are expressed predominantly in avian fibroblasts and muscle tissue. The results of these binding and mRNA expression expriments suggest that RSV-EF-II may play an important role in tissue- and host-specific expression of RSV LTR-driven gene expression. Further, we show that RSV-EF-II acts as a repressor of transcription.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
222
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
133-43
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8806494-3T3 Cells,
pubmed-meshheading:8806494-Amino Acid Sequence,
pubmed-meshheading:8806494-Animals,
pubmed-meshheading:8806494-Avian Proteins,
pubmed-meshheading:8806494-Avian Sarcoma Viruses,
pubmed-meshheading:8806494-Base Sequence,
pubmed-meshheading:8806494-Cell Line,
pubmed-meshheading:8806494-Chick Embryo,
pubmed-meshheading:8806494-Cloning, Molecular,
pubmed-meshheading:8806494-Cricetinae,
pubmed-meshheading:8806494-DNA, Viral,
pubmed-meshheading:8806494-DNA-Binding Proteins,
pubmed-meshheading:8806494-Enhancer Elements, Genetic,
pubmed-meshheading:8806494-Fibroblasts,
pubmed-meshheading:8806494-Gene Expression Regulation, Viral,
pubmed-meshheading:8806494-Mice,
pubmed-meshheading:8806494-Molecular Sequence Data,
pubmed-meshheading:8806494-Muscles,
pubmed-meshheading:8806494-Rats,
pubmed-meshheading:8806494-Sequence Homology, Amino Acid,
pubmed-meshheading:8806494-Transcription Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Cloning of Rous sarcoma virus enhancer factor genes. II. RSV-EF-II, abundantly expressed in fibroblasts and muscle tissue, binds to an octamer sequence, 5'-GTACCACC-3', in the noncoding strand of RSV enhancer.
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pubmed:affiliation |
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia 65212, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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