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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-2-28
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pubmed:databankReference |
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pubmed:abstractText |
The capacity to initiate DNA replication appears during oocyte maturation in Xenopus. Initiation of S phase is driven by several components which include active cyclin/cdk complexes. We have identified three Xenopus cyclin E clones showing 59% amino acid identity with human cyclin E. The recruitment of cyclin E mRNA, like cdk2 mRNA, into the polysomal fraction during oocyte maturation, results in the accumulation of the corresponding proteins in unfertilized eggs. Cyclin E mRNA remains polyadenylated during cleavage and anti-cyclin E antibodies detect Xlcyclin E in embryonic nuclei at this time. Cdk2 protein is necessary for the phosphorylation of radiolabelled cyclin E added to egg extracts. Radiolabelled Xlcyclin E enters interphase nuclei and, though stable through interphase and mitosis, is not associated with condensed mitotic chromatin. In egg extracts, endogenous Xlcyclin E rapidly associates with nuclei before S phase and remains nuclear throughout interphase, becoming nucleoplasmic in G2/prophase. Under conditions where initiation of replication is limiting in extracts, Xlcyclin E associates only with those nuclei that undergo S phase. These features are entirely consistent with the view that Xlcyclin E is required for initiation of S phase.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDC2-CDC28 Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/CDK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk2 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Poly A,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9533
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
109 ( Pt 6)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1173-84
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8799808-Amino Acid Sequence,
pubmed-meshheading:8799808-Animals,
pubmed-meshheading:8799808-Base Sequence,
pubmed-meshheading:8799808-CDC2-CDC28 Kinases,
pubmed-meshheading:8799808-Cell Nucleus,
pubmed-meshheading:8799808-Cloning, Molecular,
pubmed-meshheading:8799808-Cyclin-Dependent Kinase 2,
pubmed-meshheading:8799808-Cyclin-Dependent Kinases,
pubmed-meshheading:8799808-Cyclins,
pubmed-meshheading:8799808-DNA, Complementary,
pubmed-meshheading:8799808-DNA Replication,
pubmed-meshheading:8799808-Female,
pubmed-meshheading:8799808-Humans,
pubmed-meshheading:8799808-Molecular Sequence Data,
pubmed-meshheading:8799808-Oocytes,
pubmed-meshheading:8799808-Phosphorylation,
pubmed-meshheading:8799808-Poly A,
pubmed-meshheading:8799808-Protein Processing, Post-Translational,
pubmed-meshheading:8799808-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8799808-RNA, Messenger,
pubmed-meshheading:8799808-S Phase,
pubmed-meshheading:8799808-Xenopus,
pubmed-meshheading:8799808-Xenopus Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Xenopus cyclin E, a nuclear phosphoprotein, accumulates when oocytes gain the ability to initiate DNA replication.
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pubmed:affiliation |
Département de Biologie et Génétique du développement, CNRS URA 256, Université de Rennes I, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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